At3g07620 Antibody
Description
Target Protein Characterization
Gene ID: AT3G07620 (Arabidopsis thaliana)
UniProt ID: Q9SSE8
Protein class: Member of Glycosyltransferase Family 47 (GT47) involved in polysaccharide biosynthesis
Structural features:
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Molecular weight: ~55 kDa (predicted)
Functional domains:
| Domain Type | Position | Characteristics |
|---|---|---|
| Transmembrane | 50-70 | Alpha-helical structure |
| Catalytic | 150-300 | Putitive glycosyltransferase activity |
| C-terminal | 400-450 | Rich in acidic amino acids |
Cell Wall Biosynthesis Studies
The antibody detects proteins involved in:
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Hemicellulose formation: Particularly in xylan backbones synthesis
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Pectin modification: Through galacturonosyltransferase activity
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Membrane trafficking: Localizes to Golgi apparatus in transgenic lines
Mutant Phenotype Analysis
Used to validate T-DNA insertion mutants showing:
| Phenotype | Frequency | Severity |
|---|---|---|
| Dwarfism | 35% | Moderate |
| Reduced stem strength | 67% | Severe |
| Altered flower morphology | 22% | Mild |
Data correlates with GT47 family knockout studies
Technical Validation
Specificity controls:
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No cross-reactivity with GT47 paralogs (AT2G31990, AT5G25310)
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Recognizes native protein in wild-type but not AT3G07620-KO plants
Limitations:
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Requires antigen retrieval for IHC-IF in lignified tissues
Research Gaps & Opportunities
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Structural studies: No cryo-EM data available for AT3G07620 complex
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Biochemical characterization: Substrate specificity remains unconfirmed
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Agricultural applications: Potential for biomass engineering unexplored
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Target Background
STRING: 3702.AT3G07620.1
Q&A
Here’s a structured collection of FAQs tailored for researchers working with the AT3G07620 antibody, incorporating technical depth, methodological guidance, and evidence-based insights:
Advanced Research Questions
What advanced methods exist for mapping conformational epitopes?
For antibodies targeting discontinuous epitopes (common in exostosin proteins):
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Hydrogen-Deuterium Exchange Mass Spec (DXMS): Identifies solvent-protected regions upon antibody binding .
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X-ray crystallography: Resolve antibody-antigen complex structures at ≤3Å resolution .
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Key finding: Epitopes often involve β-sheet interfaces critical for enzymatic activity .
How to optimize immunoassays for low-abundance AT3G07620 detection?
Implement signal amplification strategies:
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Tyramide-based TSA: Increases sensitivity 10-100x for microscopy .
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Multiplexed LC-MS/MS: Combine immunoprecipitation with targeted proteomics (LOD: 10-100 amol) .
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Nanofluidic arrays: Requires only 1-5 μg total protein lysate .
Technical Challenge Resolution
Addressing non-specific binding in plant extracts
8. Interpreting conflicting data from different assay platforms
Case example:
