At3g42180 Antibody

Molecular Characterization of At3g42180

The At3g42180 gene encodes a putative exostosin family protein involved in glycosyltransferase activity, potentially participating in cell wall biosynthesis or modification . Key features include:

• Gene ID: AT3G42180 (Arabidopsis thaliana)

• UniProt ID: Q3EAR7

• Protein Family: Exostosin, known for roles in polysaccharide biosynthesis (e.g., heparan sulfate or plant cell wall components) .

3.1. Functional Insights

• Cell Wall Biosynthesis: At3g42180 is hypothesized to function in cell wall dynamics due to its homology to exostosin enzymes, which catalyze glycosyltransferase reactions in polysaccharide chain elongation .

• Mutant Studies: Insertional mutants of At3g42180 showed no detectable changes in leaf cell wall monosaccharide composition, suggesting functional redundancy or compensatory mechanisms in Arabidopsis .

Comparative Analysis of Exostosin Family Members

The exostosin family in Arabidopsis includes multiple paralogs, but At3g42180 is distinct in its genomic context and substrate specificity :

Technical Applications

• Antibody Validation: Specificity confirmed via immunoblotting against recombinant At3g42180 protein .

• Cross-Reactivity: No reported cross-reactivity with other Arabidopsis exostosin homologs (e.g., At5g25310) .

Implications for Broader Research

• Plant Biotechnology: Tools like the At3g42180 antibody enable studies on cell wall engineering for biofuel crops or stress-resistant varieties .

• Disease Models: Exostosin dysfunction in animals links to skeletal disorders; plant homologs may inform evolutionary studies of glycosylation pathways .