At5g25310 Antibody

Antigen Identification and Biological Context

The At5g25310 gene in Arabidopsis thaliana encodes a protein with UniProt accession Q3E7Q9. While the exact functional role of this protein remains under investigation, antibodies against plant-specific genes like At5g25310 are critical for:

• Protein localization studies: Determining spatial expression patterns in plant tissues.

• Gene function analysis: Validating knockout or overexpression phenotypes.

• Interaction studies: Identifying binding partners via immunoprecipitation or pull-down assays.

Validation and Quality Control

Commercial antibodies like At5g25310 undergo rigorous validation:

• Specificity: Verified via immunoblotting against recombinant protein and knockout controls .

• Cross-reactivity: Tested against related plant species to ensure target exclusivity .

• Batch consistency: Quality checks ensure reproducibility across lots .

Challenges and Considerations

• Limited functional data: The biological role of At5g25310 remains uncharacterized, necessitating further studies.

• Antibody validation: Researchers must perform additional controls (e.g., peptide competition, genetic knockouts) to confirm specificity .

Future Directions

• CRISPR/Cas9 integration: Pairing the antibody with gene-edited lines to validate protein absence or modification.

• Interactome mapping: Identifying binding partners via co-immunoprecipitation and mass spectrometry .