CSF2RA Human, sf9
Description
Functional Role in GM-CSF Signaling
As the alpha subunit (GM-CSFRα), CSF2RA binds GM-CSF with low affinity and partners with the beta subunit (CSF2RB) to form a high-affinity receptor complex. This interaction triggers pathways like JAK/STAT and MAPK, essential for:
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Hematopoiesis: Differentiation and proliferation of granulocytes and macrophages .
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Immune Regulation: Modulation of inflammatory responses and surfactant metabolism .
Mutations in CSF2RA are linked to pulmonary surfactant metabolism dysfunction type 4 (SMDP4), a rare lung disorder characterized by alveolar protein accumulation .
Production and Formulation
The recombinant protein is synthesized using baculovirus-mediated expression in insect cells, ensuring proper post-translational modifications. Key production details include:
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Formulation: Phosphate-buffered saline (pH 7.4) with 10% glycerol .
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Stability: Maintains activity under recommended storage conditions (-20°C to -80°C) .
4.1. Attenuation of Acute Lung Injury (ALI)
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Study Model: Csf2ra knockout (KO) mice exposed to aerosolized ricin toxin (RT) .
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Key Findings:
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Implication: CSF2RA inhibition may mitigate ALI severity by dampening inflammation .
4.2. Gene Therapy for Hereditary Pulmonary Alveolar Proteinosis (hPAP)
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Study Model: Csf2ra⁻/⁻ mice treated with lentiviral vector-corrected macrophages .
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Key Findings:
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Implication: Macrophage-targeted gene therapy is a viable strategy for hPAP .
Therapeutic Potential
CSF2RA Human, sf9 is explored for:
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Autoimmune Diseases: Acting as a decoy receptor to neutralize excess GM-CSF .
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Hematopoietic Disorders: Diagnostic tools for granulocyte/macrophage dysfunctions .
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Drug Development: Screening GM-CSF antagonists or agonists .
Limitations and Future Directions
Product Specs
The granulocyte-macrophage colony-stimulating factor receptor alpha (CSF2RA), also known as the CD116 antigen, is a protein encoded by the CSF2RA gene in humans. This receptor is crucial for the biological activity of colony-stimulating factor 2 (CSF2), a cytokine that plays a vital role in the production, differentiation, and function of granulocytes and macrophages, which are essential components of the immune system. CSF2RA is a member of the cytokine receptor family and is located on both the X and Y chromosomes in a region known as the pseudoautosomal region (PAR). The gene encoding for CSF2RA exhibits multiple transcript variants, resulting in various isoforms of the protein, some of which are bound to the cell membrane while others are soluble. Dysregulation of CSF2RA has been implicated in various diseases, including surfactant metabolism dysfunction, a condition that affects lung function.
Recombinant CSF2RA protein, expressed in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. This protein consists of 310 amino acids, spanning from position 20 to 320 (20-320a.a.), with a molecular weight of 35.9 kDa. On SDS-PAGE, the protein exhibits an apparent molecular weight of approximately 40-57 kDa due to glycosylation. The recombinant CSF2RA protein is engineered with a 9-amino acid Histidine tag (His-tag) at the C-terminus to facilitate purification using proprietary chromatographic techniques.
The CSF2RA protein is supplied as a solution with a concentration of 0.5 mg/ml. The formulation buffer consists of Phosphate Buffered Saline (PBS) at a pH of 7.4 and 10% glycerol.
For short-term storage (up to 2-4 weeks), the CSF2RA protein should be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. To further enhance long-term stability, adding a carrier protein such as 0.1% HSA (Human Serum Albumin) or BSA (Bovine Serum Albumin) is advisable. Repeated freeze-thaw cycles should be avoided to maintain protein integrity and activity.
The purity of the CSF2RA protein is determined to be greater than 90% by SDS-PAGE analysis.
The biological activity of CSF2RA is evaluated based on its capacity to inhibit the proliferation of TF1 human erythroleukemic cells in a GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) dependent manner. The ED50 value, which represents the effective concentration at which 50% inhibition is observed, is determined to be less than or equal to 10 µg/ml in the presence of 0.5 ng/ml GM-CSF.
Colony Stimulating Factor 2 Receptor Alpha Subunit, Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage), Alpha-GM-CSF Receptor, GM-CSF-R-Alpha, CD116 Antigen, GMCSFR-Alpha, GMR-Alpha, CDw116, CSF2RY, CSF2R, Granulocyte-Macrophage Colony-Stimulating Factor Receptor Subunit Alpha, Granulocyte-Macrophage Colony-Stimulating Factor Receptor Alpha Chain, GM-CSF Receptor Alpha Subunit, AlphaGMR, CSF2RAX, CSF2RAY, CSF2RX, GMCSFR, CD116, SMDP4, GMR.
ADPLIPEKSD LRTVAPASSL NVRFDSRTMN LSWDCQENTT FSKCFLTDKK NRVVEPRLSN NECSCTFREI CLHEGVTFEV HVNTSQRGFQ QKLLYPNSGR EGTAAQNFSC FIYNADLMNC TWARGPTAPR DVQYFLYIRN SKRRREIRCP YYIQDSGTHV GCHLDNLSGL TSRNYFLVNG TSREIGIQFF DSLLDTKKIE RFNPPSNVTV RCNTTHCLVR WKQPRTYQKL SYLDFQYQLD VHRKNTQPGT ENLLINVSGD LENRYNFPSS EPRAKHSVKI RAADVRILNW SSWSEAIEFG SDDGHHHHHH
Product Science Overview
The Granulocyte-Macrophage Colony-Stimulating Factor Receptor Alpha (GM-CSF Receptor Alpha), also known as CSF2RA, is a crucial component in the regulation of white blood cell production, differentiation, and function. This receptor is part of the cytokine receptor family and plays a significant role in the immune system by controlling the production and function of granulocytes and macrophages .
The GM-CSF Receptor Alpha is the alpha subunit of the heterodimeric receptor for colony-stimulating factor 2 (CSF2). It is produced in Sf9 Baculovirus cells and is a single, glycosylated polypeptide chain containing 310 amino acids, with a molecular mass of approximately 35.9 kDa . The receptor is expressed with a 9 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .
The gene encoding the GM-CSF Receptor Alpha is located in the pseudoautosomal region (PAR) of the X and Y chromosomes. This gene has multiple transcript variants, some of which are membrane-bound while others are soluble . Diseases associated with CSF2RA include surfactant metabolism dysfunction, pulmonary 4, and CSF2RA-related pulmonary surfactant metabolism dysfunction .
The recombinant form of GM-CSF Receptor Alpha has significant potential in research and therapeutic applications. It is used in studies related to immune response, hematopoiesis, and various diseases associated with the immune system. The availability of this recombinant protein enables researchers to explore its role in different biological processes and develop targeted therapies for related disorders .
The GM-CSF Receptor Alpha protein solution is formulated with phosphate-buffered saline (pH 7.4) and 10% glycerol. For short-term storage, it should be kept at 4°C if used within 2-4 weeks. For long-term storage, it is recommended to freeze the solution at -20°C with the addition of a carrier protein (0.1% HSA or BSA) to avoid multiple freeze-thaw cycles .
