Customize GNL1 Antibody

Description

Definition and Key Features of GNL1 Antibody

GNL1 antibody is a rabbit-derived polyclonal immunoglobulin designed to bind specifically to the GNL1 protein. Key attributes include:

Parameter	Details
Immunogen	Recombinant GNL1 fusion protein (e.g., Ag5203) or synthetic peptide (aa 50–100)
Target Molecular Weight	69 kDa (observed in Western blot)
Host Species	Rabbit
Reactivity	Human (validated in HeLa, MCF-7, Raji, K-562, and AGS cells)
Applications	Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF/ICC)

Research Findings and Applications

The antibody enables critical insights into GNL1’s role in oncology and cellular biology, including:

Role in Cancer Progression

GNL1 is upregulated in cancers (e.g., colon, breast, ovarian) and promotes cell survival via:

Antiapoptotic signaling: Inhibiting caspase-7/8 cleavage and modulating Bcl-2 family proteins (e.g., Bcl-xL, survivin) .
Cell cycle regulation: Inducing hyperphosphorylation of retinoblastoma protein (pRb) to drive S-phase progression .
AKT/p21 pathway activation: Stabilizing cytoplasmic p21 via AKT-mediated phosphorylation, bypassing p53-dependent growth arrest .

Mechanism	Experimental Evidence	Citation
Apoptosis inhibition	Reduced cleaved caspase-7/8 and PARP in GNL1-overexpressing HCT116 cells
Cell cycle progression	Increased BrdU incorporation and E2F1 expression in GNL1-transfected cells
p21 stabilization	Cytoplasmic retention of p21 via AKT phosphorylation in GNL1-expressing cells

Interactions and Pathway Crosstalk

GNL1 interacts with ribosomal protein S20 (RPS20), which stabilizes GNL1 and amplifies its pro-proliferative effects . Antibody-based co-immunoprecipitation (Co-IP) confirmed this interaction in HCT116 and AGS cells .

Validation and Protocol Guidelines

The antibody’s performance is validated across multiple platforms:

Tested Applications

Application	Dilution	Sample Type	Detection Method
WB	1:2000–1:12000	HeLa, MCF-7, Raji cell lysates	ECL (3 min exposure)
IHC	1:20–1:200	Human heart, skin tissue	Antigen retrieval (pH 9.0 TE buffer)
IF/ICC	1:200–1:800	MCF-7 cells	Fluorescent microscopy

Protocol Highlights

WB: Optimal detection at 1:2000–1:12000 dilution; 69 kDa band confirmed in HeLa lysates .
IHC: Requires heat-induced antigen retrieval (TE buffer, pH 9.0) for tissue samples .
IF/ICC: Compatible with MCF-7 cells; no cross-reactivity reported in tested samples .

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

GNL1 antibody; ERMO1 antibody; GBF1 antibody; At5g39500 antibody; MUL8.20ARF guanine-nucleotide exchange factor GNL1 antibody; Protein ENDOPLASMIC RETICULUM MORPHOLOGY 1 antibody; Protein GNOM-like 1 antibody

Target Names

GNL1

Uniprot No.

Q9FLY5

Target Background

Function

This antibody targets GNL1, a protein that activates ARF proteins by exchanging bound GDP for free GTP. GNL1 plays a crucial role in vesicular protein sorting and acts as the major regulator of retrograde Golgi to endoplasmic reticulum trafficking. It is also involved in the endocytosis process and may function redundantly with GNOM. GNL1 regulates vesicle trafficking required for the coordinated polar localization of auxin efflux carriers, which in turn determines the direction of auxin flow. It mediates the endocytosis of PIN2 from the plasma membrane to endosomal compartments and is required for the maintenance of endoplasmic reticulum morphology.

Gene References Into Functions

These data reveal an unanticipated level of complexity in the biology of GNL1, demonstrating that its cellular roles are correlated with cell growth. PMID: 23125314
Large ARF-GEFs of the GBF1 class perform a conserved role in endoplasmic reticulum-Golgi trafficking and secretion, which is carried out by GNL1 and GNOM in Arabidopsis. [GNL1] PMID: 17653190
GNL1 is a BFA-resistant GBF protein that functions with a BFA-sensitive ARF-GEF both at the Golgi and in selective endocytosis, but not in recycling from endosomes. [GNL1] PMID: 17653191
GNL2 encodes a protein homologous to the adenosine diphosphate-ribosylation factor-guanine nucleotide exchange factors, GNOM and GNL1, which are involved in endosomal recycling and endoplasmic reticulum-Golgi vesicular trafficking. PMID: 19686373

Database Links

KEGG: ath:AT5G39500

STRING: 3702.AT5G39500.1

UniGene: At.30340

Subcellular Location

Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Soluble and partially membrane-bound.

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GNL1 Antibody