At2g16790 Antibody

Research Context and Functional Relevance

The At2g16790 protein belongs to the shikimate kinase family, which plays a role in nitrogen assimilation and metabolic regulation in plants . In Arabidopsis, nitrogen metabolism is tightly regulated by master regulators like CCA1, GLK1, and bZIP11, which coordinate responses to nitrogen availability . The At2g16790 antibody is likely used to study:

Key Research Applications

1. Nitrogen Assimilation Pathways:

   • Investigating interactions between At2g16790 and enzymes like glutamine synthetase (GS) or asparagine synthetase (ASN1), which convert inorganic nitrogen into amino acids .

   • Analyzing feedback regulation of nitrogen storage (e.g., asparagine synthesis) in response to glutamine/glutamate levels .

2. Transgenic Plant Studies:

   • Overexpression or knockdown experiments to modulate nitrogen-use efficiency or storage capacity .

   • Monitoring protein abundance in mutants lacking nitrogen-responsive genes (e.g., CCA1, GLK1) .

Limited Publicly Available Data

Unlike well-characterized antibodies in human immunology (e.g., SARS-CoV-2 antibodies or IgG production markers ), the At2g16790 antibody lacks extensive peer-reviewed validation. This contrasts with databases like PLAbDab, which curate antibody sequences and structures for therapeutic and research purposes .

Potential Off-Target Reactivity

While not reported for At2g16790, commercial plant antibodies sometimes exhibit nonspecific binding (e.g., angiotensin II AT2 receptor antibodies ). Researchers should validate results with orthogonal methods (e.g., CRISPR knockouts or RNAi).

Future Directions

The At2g16790 antibody could enable deeper insights into nitrogen metabolism in crops, particularly under nitrogen-limiting conditions. Potential areas include:

1. Agronomic Applications:

   • Engineering plants with enhanced nitrogen assimilation for improved yield or drought tolerance.

2. Omics Integration:

   • Combining antibody-based protein quantification with metabolomics to map nitrogen flux pathways.