Goat Anti-Mouse IgG(H+L) Antibody;Biotin conjugated

Molecular Composition and Characteristics

This antibody contains the following key components:

Mechanism of Signal Amplification

The biotin-avidin system enhances detection sensitivity through two primary methods:

Avidin-Biotin Complex (ABC) Method

• Three-step process: Primary antibody → Biotinylated secondary → Avidin-enzyme complex

• Generates large enzyme clusters for chromogenic/chemiluminescent signals

Labeled Streptavidin Biotin (LSAB) Method

• Uses pre-formed streptavidin-enzyme conjugates

• Provides 8x greater sensitivity than ABC method

• Preferred for tissue penetration-limited applications

Key Applications and Performance Data

Cross-Reactivity Management Strategies

Different formulations address species interference:

Stability and Lot Consistency

• Thermal Stability: Maintains activity after 5 freeze-thaw cycles

• Shelf Life: 24 months at -20°C with <15% activity loss

• Lot Validation:

  • ELISA titers ≥1:64,000 against mouse IgG

  • <5% inter-lot variability in recombinant versions

Research Case Studies

1. Neurofilament Detection: Identified NEFH in rat brain sections using 10 μg/mL secondary antibody with 92% staining specificity

2. Cancer Diagnostics: Enabled PCNA visualization in human intestinal tumors at 1:500 dilution, improving SNR by 40% vs fluorescent labels

3. High-Throughput Screening: Processed 5,000 ELISA samples/day using biotin-streptavidin-HRP system