GC4 Antibody

GC4 Antibody Targeting N-Cadherin

Target: N-Cadherin (neural cadherin), a transmembrane glycoprotein involved in cell adhesion and signaling.
Host/Clonality: Mouse monoclonal (IgG1) .
Applications:

• Western blotting (WB): Validated for detecting N-Cadherin in human, mouse, rat, rabbit, and chicken tissues .

• Immunocytochemistry (ICC/IF): Used to visualize N-Cadherin localization in non-permeabilized cells .

• Functional studies: Demonstrated that CS (chondroitin sulfate)-mediated N-Cadherin/β-catenin signaling enhances breast cancer cell invasion .

Key Findings:

• CS binding to N-Cadherin triggers caveolae-mediated endocytosis, leading to β-catenin nuclear translocation and transcriptional activation of genes like MMP9 (matrix metalloproteinase-9) .

• Inhibition of N-Cadherin with GC4 antibodies blocks CS-induced invasion in basal-like breast cancer cells (e.g., BT-549) .

GC4 Antibody Targeting NDRG1

Target: NDRG1 (N-myc downstream-regulated gene 1), a stress-responsive protein implicated in tumor suppression.
Host/Clonality: Chicken polyclonal .
Applications:

• Western blotting (WB): Detects NDRG1 in human and mouse samples .
  Product Specifications:

• Source: E. coli-derived fusion protein.

• Purity: Immunoaffinity-purified IgY.

• Storage: -20°C for long-term storage .

Limitations: Limited functional data in peer-reviewed studies; primarily a commercial product .

GC4 Antibody Targeting Fibrin

Target: Fibrin, a fibrous protein critical for blood clot formation.
Host/Clonality: Mouse monoclonal (IgG1) .
Applications:

• Thrombus imaging: Radiolabeled GC4 (e.g., with indium-111) localizes to fibrin in experimental thrombi, enabling scintigraphic detection .
  Key Findings:

• GC4 binds specifically to plasmin-digested fibrinogen and fibrin, with >70% immunoreactivity .

• No cross-reactivity with T2G1s (another anti-fibrin antibody), indicating distinct epitopes .

GC4 Antibody Targeting Glioblastoma (GBM) Tumor Cells

Target: GBM tumor sphere cells, enriched for cancer stem cells.
Host/Clonality: Human single-chain variable fragment (scFv) .
Applications:

• Internalization studies: GC4 scFv internalizes into GBM cells, enabling targeted drug delivery.

• Proliferation inhibition: Full-length GC4 IgG1 suppresses GBM tumor sphere cell self-renewal and proliferation .

Key Findings:

• GC4 scFv binds with high affinity (apparent Kd ~100 nM) to GBM cells .

• GC4 IgG1 inhibits tumor sphere formation in vitro, suggesting therapeutic potential against glioblastoma recurrence .

Comparative Analysis of GC4 Antibodies