GPR107 Antibody, FITC conjugated
Description
Overview of GPR107 Antibodies
GPR107 (G protein-coupled receptor 107) is an orphan receptor involved in intracellular trafficking, toxin entry, and cardiovascular regulation. Antibodies targeting GPR107 are primarily polyclonal (rabbit) and are used in:
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Western blot (WB): Detection of denatured protein (60–67 kDa) .
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Immunofluorescence (IF)/ICC: Localization studies in HEK-293 cells and tissue sections .
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Immunohistochemistry (IHC): Paraffin-embedded or frozen tissue analysis .
Potential Applications for FITC-Conjugated GPR107 Antibodies
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Live-cell imaging: Tracking GPR107 localization in real-time.
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Co-localization studies: Identifying interactions with Golgi/TGN markers .
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Flow cytometry: Quantifying GPR107 expression on cell surfaces.
Recommended Dilutions for Unconjugated Antibodies (Reference for FITC)
Role in Toxin Entry
GPR107 is critical for retrograde transport from the Golgi to the endoplasmic reticulum (ER). It is required for the entry of Pseudomonas aeruginosa exotoxin A (PE) and Campylobacter jejuni CDT toxins, acting as a host factor in toxin trafficking .
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Mechanism: Cleaved by furin, GPR107 facilitates toxin retrograde transport, potentially via G-protein interactions .
Cardiovascular Regulation
GPR107 is proposed as a receptor for neuronostatin, a peptide involved in central cardiovascular control. This interaction may modulate blood pressure and heart rate .
Recommendations for Obtaining FITC-Conjugated GPR107 Antibodies
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Custom Synthesis: Contact suppliers like Proteintech, Boster Bio, or R&D Systems to inquire about conjugation services.
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In-House Conjugation: Use FITC labeling kits (e.g., Thermo Fisher Scientific) with validated GPR107 antibodies (e.g., DF4939 , 25076-1-AP ).
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Validation: Test conjugated antibodies in IF/ICC to confirm specificity and fluorescence efficiency.
Research Gaps and Future Directions
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Lack of Commercial FITC-Conjugated Products: No vendors explicitly list FITC-labeled GPR107 antibodies, necessitating custom synthesis.
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Unexplored Applications: FITC conjugation could enable time-lapse studies of GPR107 dynamics in toxin trafficking or cardiovascular signaling.
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- The N-terminal region of GPR107 is crucial for its biological function. GPR107 may be one of the receptors that associate with G-proteins to regulate intracellular vesicular transport. PMID: 25031321
- GPR107 is a promising candidate receptor for neuronostatin. Neuronostatin, interacting with GPR107, may play a significant role in the central control of cardiovascular function. PMID: 22933024
