GPRC5A Antibody
Description
Flow Cytometry
The antibody is optimized for detecting GPRC5A on the surface of live or fixed cells. In studies using A549 lung carcinoma cells, it successfully differentiated GPRC5A-expressing cells from negative controls, with a staining efficiency exceeding 90% .
Immunohistochemistry (IHC)
GPRC5A Antibody has been used to analyze tumor specimens, revealing reduced expression in lung adenocarcinoma (LUAD) compared to normal lung tissue. This correlates with enhanced tumor aggressiveness, as observed in GPRC5A knockout mice .
Western Blotting
While not explicitly validated by the manufacturer, independent studies have employed the antibody to confirm GPRC5A downregulation in NSCLC patient samples, supporting its utility in protein-level analysis .
Tumor Suppression in Lung Cancer
GPRC5A functions as a tumor suppressor in lung tissues, with its loss linked to spontaneous tumor formation in knockout mice (76% incidence vs. 10% in wild-type) . The antibody has been instrumental in demonstrating that GPRC5A expression inversely correlates with immune cell infiltration (e.g., CD8+ T cells) and tumor progression in NSCLC patients .
Dual Role in Cancer
Contrary to its tumor-suppressive role in lung cancer, GPRC5A overexpression promotes metastasis in esophageal squamous cell carcinoma (ESCC). Studies using this antibody revealed that GPRC5A-high ESCC cells exhibit enhanced lung colonization and survival, mimicking trophoblast implantation mechanisms .
Table 1: GPRC5A Expression Across Cancer Types
Table 2: GPRC5A Antibody Performance Metrics
| Assay | Sensitivity | Specificity | Signal-to-Noise Ratio |
|---|---|---|---|
| Flow Cytometry (A549) | >90% | >95% | 8:1 |
| IHC (LUAD vs. normal) | 85% | 92% | N/A (qualitative) |
Product Specs
Target Background
- GPRC5A upregulation in pancreatic cancer (PaCa) has been linked to enhanced drug resistance in PaCa cells. PMID: 29949874
- Downregulated GPRC5A expression during lung tumorigenesis enhances any transcriptional stimulation through an active translational status. PMID: 27273304
- Research indicates a novel role for GPRC5A in regulating ITGB1-mediated cell adhesion and its downstream signaling, suggesting potential significance in human epithelial cancers. PMID: 27715394
- miR-204 inhibits cell proliferation in gastric cancer by targeting CKS1B, CXCL1, and GPRC5A. PMID: 29283424
- p53 overexpression and GPRC5A induction significantly inhibited tumor cell viability and induced apoptosis. PMID: 28849235
- GPRC5A has been identified as a potential oncogene in pancreatic ductal adenocarcinoma cells, and its upregulation by gemcitabine is facilitated by HuR. PMID: 27415424
- These findings suggest that RAI3 plays a crucial role in adipogenesis of hASCs and may hold potential for future applications. PMID: 28870805
- This study highlights genomic alterations representing early events in the development of Kras mutant LUAD following Gprc5a loss and tobacco carcinogen exposure. PMID: 28653505
- Suppression of GPRC5a leads to decreased cell growth, proliferation, and migration in pancreatic cancer cell lines through a STAT3-modulated pathway, independent of ERK activation. PMID: 28114355
- Our findings suggest that GPRC5A acts as a tumor suppressor in breast cancer cells, inhibiting EGFR and its downstream pathway. PMID: 27599526
- Elevated levels of GPRC5A have been implicated in the progression of gastric cancer. PMID: 26227221
- Results demonstrate how GPRC5A deficiency leads to dysregulated EGFR and STAT3 signaling, contributing to lung tumorigenesis. PMID: 25744720
- EGFR interacts with GPRC5A and phosphorylates it in two conserved double-tyrosine motifs, Y317/Y320 and Y347/Y350, at the C-terminal tail of GPRC5A. PMID: 25311788
- The interaction of miR-103a-3p with each of the two 5' UTR targets reduces the expression levels of both GPRC5A mRNA and GPRC5A protein in one normal epithelial and two pancreatic cancer cell lines. PMID: 24984703
- RAI3 may contribute to the malignant progression of hepatocellular carcinoma. PMID: 23632812
- Data indicate that in mammary tumors, the mRNA expression of GPRC5A significantly correlated with that of BRCA1. PMID: 24470238
- Decreased GPRC5A expression is associated with non-small cell lung cancers and lung inflammation. PMID: 23154545
- Loss of GPRC5A is associated with lung adenocarcinomas. PMID: 20563252
- RAI3 is a cell growth-promoting gene and a novel P53 transcriptional target. PMID: 15659406
- This evidence elucidates one of the mechanisms by which GPRC5A regulates cell growth in certain cancer cell lines. PMID: 17055459
- Gprc5a functions as a tumor suppressor in mouse lung, and human GPRC5A may share this property. PMID: 18000218
- Mechanisms underlying the induction of the putative human tumor suppressor GPRC5A are reported. PMID: 19279407
- Analysis of RAI3 expression in normal and cancerous human breast tissue at both the mRNA and protein levels has been conducted. PMID: 19552806
