At4g03950 Antibody

Introduction to At4g03950 Antibody

The At4g03950 Antibody is a polyclonal rabbit-derived antibody designed to detect the Arabidopsis thaliana protein encoded by the AT4G03950 gene. This antibody is primarily used in molecular biology research to study gene expression, protein localization, and functional characterization in plant models. Its specificity allows researchers to analyze the nucleotide/sugar transporter family protein, which is implicated in cellular transport mechanisms and stress responses .

Applications in Research

The At4g03950 Antibody is utilized in diverse experimental approaches:

Protein Detection and Localization

• Western Blot (WB): Detects the target protein in Arabidopsis extracts, enabling quantification of expression levels under specific conditions .

• ELISA: Used for high-throughput screening of At4g03950 protein abundance in samples .

Functional and Expression Studies

Research has shown that AT4G03950 encodes a nucleotide/sugar transporter, which is upregulated in response to epigenetic stress or environmental challenges . For example:

• Epigenetic Variation: Overexpression of AT4G03950 was observed in Arabidopsis lines with induced epigenetic changes, suggesting its role in stress adaptation .

• Transporter Activity: The protein belongs to the solute carrier family 35 (SLC35), facilitating the transport of nucleotides or sugars across membranes .

Gene Expression Patterns

Protein Function

• Role in Transport: The encoded transporter is critical for cellular homeostasis, potentially regulating nucleotide availability during growth or stress .

• Interaction Networks: While direct interactors are not explicitly detailed, its classification in the SLC35 family implies functional overlap with other nucleotide transporters .