GRIK3 Antibody
Description
GRIK3 Antibody: Definition and Molecular Context
GRIK3 antibodies target the GluK3 subunit of kainate receptors, which are ligand-gated ion channels activated by glutamate. These receptors mediate excitatory neurotransmission and are linked to synaptic plasticity, learning, and memory . In cancer biology, GRIK3 exhibits dual roles:
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Oncogenic: Promotes proliferation, migration, and epithelial-mesenchymal transition (EMT) in breast and gastric cancers .
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Tumor-suppressive: Inhibits Wnt/β-catenin signaling and metastasis in non-small cell lung cancer (NSCLC) .
Key Applications of GRIK3 Antibodies
GRIK3 antibodies are widely used in:
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Western Blot (WB): Detects GRIK3 at ~104 kDa in human cell lysates (e.g., HEK293) .
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Immunohistochemistry (IHC): Identifies overexpression in breast (vs. adjacent tissues) and gastric cancer tissues .
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Immunofluorescence (IF): Localizes GRIK3 in neuronal cells (e.g., hippocampal pyramidal layers) and cancer cell lines .
Breast Cancer
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Expression: Elevated in tumor tissues compared to adjacent normal tissues (P < 0.001) .
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Prognosis: High GRIK3 correlates with poor survival (HR = 2.1; P < 0.001) .
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Mechanism: Induces EMT via SPDEF/CDH1 suppression and β-catenin nuclear translocation .
Gastric Cancer
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Association: Overexpression linked to lymph node metastasis (LN) and advanced TNM stage (P = 0.027) .
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Survival: High GRIK3 predicts worse outcomes (HR = 1.87; 95% CI: 1.12–3.11) .
Non-Small Cell Lung Cancer
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Role: Acts as a tumor suppressor; low expression correlates with recurrence and poor survival .
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Pathway: Downregulates UBE2C/CDK1 to inhibit Wnt/β-catenin signaling .
Table 2: GRIK3 Expression Across Cancers
Mechanistic Insights
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EMT Regulation: In breast cancer, GRIK3 reduces epithelial markers (e.g., CDH1) and increases mesenchymal markers (e.g., Vimentin) .
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Wnt Signaling: In NSCLC, GRIK3 deficiency elevates UBE2C and CDK1, activating Wnt-3a/β-catenin to drive proliferation .
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Therapeutic Targeting: Blocking Wnt with FH535 reverses GRIK3-mediated tumor suppression .
Table 3: Signaling Pathways Modulated by GRIK3
| Pathway | Biological Effect | Cancer Type | Reference |
|---|---|---|---|
| EMT | Enhances migration and invasion | Breast | |
| Wnt/β-catenin | Suppresses tumor growth and metastasis | NSCLC | |
| Neuroactive ligand-receptor | Promotes LN metastasis | Gastric |
Future Directions
Product Specs
Target Background
- Additional experiments demonstrated that lymph node metastasis tissues exhibited higher GRIK3 expression compared to their matched primary gastric cancer (GC) tissues. These findings suggest that elevated GRIK3 expression could serve as an independent prognostic biomarker and a potential therapeutic target for GC patients. PMID: 28631555
- GRIK3 expression is significantly downregulated in human masticatory mucosa during wound healing. PMID: 28005267
- A deletion on chromosome 1p34.3, encompassing GRIK3, has been implicated in developmental delay. This finding further underscores the clinical significance of GRIK family glutamate receptors in the pathogenesis of developmental delay. PMID: 24449200
- Analyses revealed that the Ser310Ala polymorphism of the GRIK3 gene is not associated with alcohol dependence or any of its subgroups. PMID: 23006490
- Our findings suggest that the analyzed polymorphisms of ANKK1 and ADH4 could play a significant role in the pathogenesis of alcohol abuse. PMID: 22232963
- Some support (at a nominal significance level of 0.05/0.01) was found for an association between GRIK3 and depression. PMID: 20052609
- Our study suggests a potential role for GRIK3 in the susceptibility to schizophrenia in the Indian population. PMID: 19921975
- An association was observed between the ionotropic glutamate receptor kainate 3 (GRIK3) ser310ala polymorphism and schizophrenia. PMID: 11986986
- These findings are suggestive of a significant role of GRIK3 polymorphism in the pathogenesis of delirium tremens in alcohol-dependent individuals. PMID: 16314883
- The findings do not appear to support the hypothesis of the role of this polymorphism in the pathogenesis of alcoholism. PMID: 16356644
- Significant linkage disequilibrium (LD) was indicated by preferential maternal transmission of the GluR7 S310 allele to recurrent major depressive disorder patients (P = 0.012), but not to bipolar I disorder (BPI) patients (P = 1.00). PMID: 16958029
- While not conclusive, the present results support the need for more extensive investigations of GRIK3, given its potential role in mediating risk for schizophrenia. PMID: 19022628
- Homozygosity for the functional GRIK3 Ser310Ala polymorphism is associated with higher scores in harm avoidance and respective subscales of personality traits. PMID: 19221446
Customer Reviews
Applications : Western blot
Sample type: Human Cells
Review: CircASXL1 knockdown-mediated repression on the protein expression of GRIK3 was restrained by miR-1205 inhibitor in SW480 and SW620 cells.
