GH Denis

GH Denis Recombinant
Shipped with Ice Packs
In Stock

Description

Clarification of Terminology

The phrase "GH Denis" does not correspond to any identifiable compound in peer-reviewed studies or pharmacological databases. Possible interpretations include:

InterpretationRelevance to Search Results
Growth Hormone (GH) + DenisGerald V. Denis, PhD, studies GH-related pathways (e.g., BET proteins, inflammation, and cancer).
Ghrelin (GH secretagogue) + DenisGhrelin/GHS-R axis linked to tumor migration; Denis’s work on metastasis in breast cancer.
Hypothetical CompoundNo evidence in provided sources; may refer to small-molecule inhibitors (e.g., BM001) targeting GH/IGF1 axis.

Relevant Research by Gerald V. Denis

Gerald Denis investigates chromatin regulation, inflammation, and cancer. Key findings include:

BET Bromodomains and Cancer

  • Role in Inflammation: BET proteins (BRD2, BRD3, BRD4) co-activate NF-κB-regulated pro-inflammatory cytokines. Inhibition reduces insulin resistance in obesity .

  • Cancer Implications:

    • Breast Cancer: BET proteins link metabolism, inflammation, and progression in African American women .

    • Immune Microenvironment: BET proteins regulate cytokines/chemokines in immune cells, driving chemoresistance and metastasis .

GH/IGF1 Axis Inhibitors

Denis’s work intersects with studies targeting the GH/IGF1 axis, though not directly. For example:

  • BM001: A small molecule inhibiting GHR synthesis, reducing IGF1 levels (IC50: 10–30 nM in cancer cells) and tumor growth in xenograft models .

GH-Related Compounds and Pathways

While "GH Denis" is undefined, the search results highlight critical GH-associated research:

Ghrelin/GHS-R Axis

  • Astrocytoma Migration: Ghrelin activates GHS-R, promoting cell motility via Rac1 and MMP activity. Overexpression correlates with high-grade CNS tumors .

  • Endogenous Production: Ghrelin is acylated and binds GHS-R; des-acyl ghrelin may exert anti-apoptotic effects via unknown receptors .

Therapeutic Inhibitors

CompoundTargetMechanismIC50Clinical Relevance
BM001GHRInhibits synthesis10–30 nMReduces IGF1, liver weight in mice
JQ1BET proteinsBlocks acetylationN/AAnti-inflammatory; safety concerns in HIV latency

Product Specs

Introduction
Growth Hormone (GH) belongs to the prolactin family of hormones, which are crucial for growth regulation. The GH gene, along with four related genes, forms the GH locus on chromosome 17. These genes share the same transcriptional orientation, suggesting evolution through gene duplication. A high degree of sequence identity exists among the five genes. Alternative splicing further diversifies the five GHs by generating additional isoforms, potentially leading to specialized functions. This specific family member is expressed in the pituitary gland but not in placental tissue, unlike the other four genes in the GH locus. Mutations or deletions in this gene can result in GH deficiency, leading to short stature.
Description
Recombinant GH Denis, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 190 amino acids. It has a molecular weight of 21.81 kDa. The purification process of GH involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder
Formulation
The protein was lyophilized from a 1 mg/ml solution containing 0.0045 mM NaHCO3.
Solubility
Reconstitute the lyophilized GH Denis in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted into other aqueous solutions as needed.
Stability
Lyophilized GH Denis remains stable at room temperature for up to 3 weeks. However, for long-term storage, it should be kept desiccated at a temperature below -18°C. After reconstitution, GH Denis should be stored at 4°C for 2-7 days. For longer storage periods, freeze at -18°C. To enhance stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity of GH Denis is greater than 97.0%, as determined by the following methods: (a) Size Exclusion Chromatography - High Performance Liquid Chromatography (SEC-HPLC) (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
Synonyms

GH1, GH, GHN, GH-N, hGH-N,Pituitary GH, GH 1.

Source
Escherichia Coli.

Q&A

What is the GHAFOSIM model and how is it applied in forest research?

The GHAFOSIM model is a growth and yield model developed for analyzing permanent sample plot (PSP) data from mixed tropical forests. Developed from Ghana old series permanent sample plots, this deterministic empirical model employs diameter class projection methods with matrix algebra formulations to predict forest growth trajectories .

Methodologically, researchers apply GHAFOSIM through:

  • Automatic updating of transition matrices from PSP data

  • Integration of different crown classes and stand density interactions

  • Application of both classical and matrix algebra formulations

  • Implementation in both stand table and simulated logging modes

The model is particularly valuable for aggregated projections in the early stages of forest management or for broader sectoral overviews of potential yield when detailed data may be limited .

How does one interpret transition matrices in forest growth modeling?

Transition matrices represent the mathematical foundation for many forest growth models, including the GHAFOSIM system. These matrices capture the probability of trees moving between diameter classes over specific time periods .

To effectively interpret transition matrices:

  • Understand the underlying mathematical framework (Usher, Markov, or other formulations)

  • Recognize that diagonal elements represent trees remaining in the same class

  • Elements above the diagonal represent growth into larger classes

  • Elements below the diagonal (typically zeros) would represent regression to smaller classes

  • Examine how matrices tend toward equilibrium distributions over time

The GHAFOSIM model demonstrates how projected stand tables evolve toward an equilibrium distribution after approximately 250 years, providing insights into long-term forest dynamics under stable conditions .

What data requirements are necessary for implementing models like GHAFOSIM?

Implementing forest growth models requires comprehensive data collection and preparation. Based on the GHAFOSIM example, researchers need :

  • Multi-year permanent sample plot (PSP) measurements

  • Tree diameter measurements at consistent heights

  • Species identification for each measured tree

  • Crown class categorization for more refined models

  • Records of mortality and recruitment events

  • Spatial coordinates for competition index calculations

The data preparation process involves:

  • Error checking and validation

  • Conversion of incompatible measurements to a common basis

  • Merging multi-year datasets

  • Transformation between year-per-record and multi-year record formats

  • Calculation of stand-level statistics and competition indices

This methodical data preparation is essential for developing reliable transition matrices and initial stand tables .

How can experimental design principles improve forest growth modeling studies?

Effective experimental design is fundamental to developing reliable forest growth models. Researchers should implement a structured approach following these methodological principles :

  • Clear problem formulation and hypothesis development

  • Structured design construction with proper randomization

  • Determination of required sample sizes and replication levels

  • Appropriate model selection based on research objectives

  • Systematic data collection with standardized protocols

  • Rigorous analysis using appropriate statistical techniques

  • Sample size determination

  • Assignment of experimental units to treatment combinations

  • Selection of appropriate treatment factor combinations

  • Error control mechanisms

Such considerations help researchers move beyond simple data collection to developing robust, reproducible studies that address specific research questions about forest dynamics .

What methods exist for handling contradictory or anomalous data in long-term forestry studies?

Long-term forestry studies inevitably encounter contradictory or anomalous data. Methodological approaches for addressing these issues include :

  • Data validation protocols:

    • Systematic error checking for measurement inconsistencies

    • Cross-verification between multiple data sources

    • Graphical examination of growth patterns for biological plausibility

  • Statistical approaches:

    • Robust regression techniques less sensitive to outliers

    • Transformation of variables to address non-normality (e.g., log transformation of increment data)

    • Application of mixed models that account for random effects

  • Decision frameworks:

    • Established criteria for data exclusion based on biological impossibility

    • Documentation of all excluded observations and justification

    • Sensitivity analysis to assess impact of questionable data points

The analysis should maintain a balance between data integrity and representativeness, recognizing that some anomalies may represent important ecological phenomena rather than measurement errors .

How should researchers approach the integration of spatial factors in forest growth models?

Integrating spatial factors into forest growth models requires methodological approaches that address tree-to-tree interactions and environmental heterogeneity :

  • Competition index calculation methods:

    • Spatial indices based on distance-dependent measures

    • Competition influence overlap calculations

    • Overtopping basal area determinations

  • Spatial data preparation techniques:

    • Creation of plot maps from coordinate data

    • Calculation of nearest neighbor statistics

    • Edge correction methods for boundary effects

  • Model integration approaches:

    • Incorporation of spatial competition into increment functions

    • Development of spatially-explicit mortality models

    • Refinement of recruitment patterns based on gap dynamics

Researchers must recognize that spatial factors often show weak residual covariance with competition once diameter has been accounted for, necessitating careful statistical approaches to isolate these effects .

What statistical transformations are most appropriate for analyzing forest growth data?

Forest growth data often violates assumptions of standard statistical methods, requiring appropriate transformations. Key methodological approaches include :

  • Log transformation of diameter increment data:

    • Normalizes typically skewed increment distributions

    • Stabilizes variance across diameter classes

    • Creates more linear relationships with predictor variables

  • Weibull distribution fitting:

    • Provides flexible characterization of growth rate distributions

    • Enables parameter estimation through order statistics

    • Allows for species and crown class-specific growth patterns

  • Logistic transformations for mortality data:

    • Converts bounded probability values to unbounded scale

    • Enables standard regression techniques for binary outcomes

    • Facilitates interpretation of size and competition effects

The choice of transformation should be guided by both statistical considerations and the underlying biological processes. For example, the GHAFOSIM model uses Weibull coefficients (ALPHA and BETA) to characterize diameter increment distributions by species and crown classes .

How can researchers effectively analyze mortality patterns in forest dynamics studies?

Analyzing mortality patterns requires specialized approaches due to the binary nature of mortality data and often low mortality rates. Methodological approaches should include :

  • Standardization for variable measurement periods:

    • Calculate annualized mortality rates

    • Account for exposure time in models

    • Use appropriate aggregation methods

  • Statistical modeling techniques:

    • Apply logistic regression for binary mortality data

    • Calculate confidence limits using binomial probability theory

    • Test for species differences in mortality patterns

  • Predictor variable selection:

    • Examine size-dependent mortality functions

    • Test competition effects on mortality probability

    • Consider interaction terms between size and competition

A structured approach to mortality analysis, as demonstrated in the database structure for mortality data from Ghana old-series PSPs, enables researchers to derive robust mortality functions for inclusion in growth models .

What methods are recommended for analyzing recruitment processes in forest ecosystems?

Recruitment analysis presents unique challenges due to its episodic nature and relationship to stand conditions. Based on methodological approaches from forest research, researchers should :

  • Prepare appropriate databases:

    • Develop systems for tracking ingrowth into minimum diameter classes

    • Calculate recruitment rates per unit area and time

    • Relate recruitment to stand structure variables

  • Apply statistical techniques:

    • Use zero-inflated models for datasets with many periods of no recruitment

    • Implement lag effects to account for delayed responses to stand conditions

    • Develop species-specific recruitment functions

  • Consider ecological factors:

    • Analyze recruitment in relation to canopy gaps

    • Account for seed source availability

    • Incorporate disturbance history variables

The preparation of databases of gross recruitment from raw PSP data, as mentioned in the GHAFOSIM documentation, provides a foundation for these analytical approaches .

What methods exist for incorporating competition effects in forest growth models?

Competition among trees fundamentally influences growth patterns and requires careful modeling approaches. Methodological techniques include :

  • Competition index calculation:

    • Spatial indices based on neighbor distances and sizes

    • Competition influence overlap measurements

    • Overtopping basal area calculations

    • Non-spatial stand density measures

  • Statistical integration:

    • Regression analysis relating increment to competition measures

    • Inclusion of diameter-competition interactions

    • Separate models by crown class to capture dominance effects

  • Model implementation approaches:

    • Direct modification of increment functions

    • Adjustment of transition probabilities in matrix models

    • Density-dependent mortality functions

When implementing these approaches, researchers should be aware of the "weak residual covariance with competition once diameter has been accounted for," necessitating careful model specification and validation .

How can researchers validate and calibrate forest growth models effectively?

Validation and calibration are essential steps in developing reliable forest growth models. Methodological approaches should include :

  • Data partitioning strategies:

    • Reserve independent datasets for validation

    • Use temporal splits to test predictive ability

    • Implement cross-validation for robust assessment

  • Statistical validation techniques:

    • Calculate prediction bias and precision metrics

    • Evaluate goodness-of-fit across diameter classes

    • Compare observed versus predicted stand structures

  • Calibration methods:

    • Parameter optimization using observed data

    • Bayesian updating of model parameters

    • Sensitivity analysis to identify key parameters

  • Validation criteria:

    • Model must possess objectivity in measurement and scoring

    • Reliability through consistent measurements

    • Validity in measuring what it is intended to measure

Effective validation requires balancing statistical rigor with practical relevance, ensuring models perform well both mathematically and when applied to real forest management scenarios .

How can Denis Grant's research on chemical toxicants inform experimental design in environmental toxicology?

Denis Grant's research on the molecular mechanisms underlying pathological consequences of chemical toxicants, particularly polycyclic aromatic hydrocarbons (PAHs), provides valuable methodological insights for environmental toxicology research :

  • Experimental design approaches:

    • Use of genetically modified animal models to isolate specific mechanisms

    • Implementation of controlled exposure studies with varying dose levels

    • Application of transcriptomic and proteomic analyses to identify response pathways

  • Mechanistic investigation methods:

    • Study of bioactivation pathways for environmental chemicals

    • Analysis of cytochrome P450 (CYP) enzymes' role in converting PAHs to toxic metabolites

    • Investigation of the aryl hydrocarbon receptor (AHR) signaling pathway

  • Risk assessment methodologies:

    • Integration of pharmacokinetic data with molecular effects

    • Examination of dose-response relationships at the molecular level

    • Consideration of genetic factors affecting toxicant disposition and risk

This research demonstrates how experimental designs must account for complex biochemical pathways, genetic factors, and dose-dependent effects when studying environmental toxicants .

What methodological considerations are important when designing experiments with R for forest research?

Designing experiments with R for forest research requires careful consideration of statistical design principles and software implementation. Key methodological aspects include :

  • Experimental design selection:

    • Match design to research objectives

    • Consider error control mechanisms appropriate for forest environments

    • Determine required sample sizes and replication

  • R implementation strategies:

    • Utilize R code to create and analyze experimental designs

    • Implement appropriate randomization procedures

    • Develop functions for specific forest research applications

  • Model fitting approaches:

    • Select models appropriate for the experimental data

    • Implement mixed-effects models for hierarchical data structures

    • Address assumptions through appropriate transformations

  • Results interpretation frameworks:

    • Connect statistical outputs to research questions

    • Present results in meaningful ways to answer research questions

    • Use graphical methods to communicate complex relationships

Proper experimental design with R requires connecting research objectives to design selection, executing appropriate randomization, fitting appropriate models, and interpreting results in ways that address the original research questions .

How should researchers approach the analysis of permanent sample plot data for long-term ecological studies?

Analysis of permanent sample plot (PSP) data for long-term ecological studies requires structured methodological approaches to address temporal dependencies and changing environmental conditions :

  • Data preparation techniques:

    • Error checking and consistency verification

    • Handling of missing trees and measurement errors

    • Standardization of measurements across time periods

  • Growth analysis methods:

    • Calculation of diameter and basal area increments

    • Determination of mortality rates accounting for observation periods

    • Quantification of recruitment patterns over time

  • Advanced statistical approaches:

    • Time series analysis of growth patterns

    • Mixed-effects models to account for plot-level random effects

    • Non-linear growth functions fitted to long-term trajectories

  • Integration with environmental data:

    • Correlation of growth patterns with climate records

    • Analysis of disturbance effects on stand dynamics

    • Assessment of long-term trends in productivity

These methodological approaches provide a foundation for transforming raw PSP data into meaningful insights about forest dynamics, enabling both retrospective analysis and predictive modeling of future conditions .

Product Science Overview

Introduction

Growth hormone (GH) is a member of the prolactin family of hormones, which play a crucial role in growth control. The gene responsible for GH, along with four other related genes, is located at the GH locus on chromosome 17. These genes share a high degree of sequence identity, and alternative splicing generates additional isoforms, leading to further diversity and specialization. GH Denis Recombinant is expressed in the pituitary gland but not in placental tissue, unlike the other genes in the GH locus .

Production

GH Denis Recombinant is produced in Escherichia coli (E. coli) bacteria. The production process involves inserting the gene encoding the human growth hormone into the bacterial DNA. The bacteria then use this genetic information to produce the GH protein. The resulting protein is a single, non-glycosylated polypeptide chain containing 190 amino acids and has a molecular mass of 21,810 Daltons .

The protein is purified using proprietary chromatographic techniques to ensure high purity and quality. The final product is a sterile, filtered, white lyophilized (freeze-dried) powder .

Stability and Storage

Lyophilized GH Denis Recombinant is stable at room temperature for up to three weeks. However, for long-term storage, it should be kept desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use. It is recommended to add a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), to prevent freeze-thaw cycles .

Applications

GH Denis Recombinant is primarily used for laboratory research purposes. It is not intended for use as a drug, agricultural or pesticidal product, food additive, or household chemical .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
© Copyright 2024 Thebiotek. All Rights Reserved.