GRXS12 Antibody

GRXS12 Protein Characteristics

GRXS12 belongs to the class I glutaredoxin family characterized by:

• Active site motif: CPYC or CGFS (varies by isoform)

• Molecular function: Catalyzes deglutathionylation reactions using glutathione (GSH) as cofactor

• Subcellular localization: Primarily plastid-targeted, with roles in chloroplast redox regulation

• Key structural feature: Contains N-terminal chloroplast transit peptides for organelle targeting

Key Research Applications of GRXS12 Antibody

Critical Research Findings Using GRXS12-Specific Tools

Viral Defense Mechanisms

• Transgenic Nicotiana benthamiana expressing CcGRXS12 showed:

  • 63% reduction in PMMoV-I viral coat protein accumulation vs controls

  • Upregulation of SA pathway markers (PR1, PR2) with simultaneous JA/ET pathway suppression

Redox Regulation Dynamics

• GRXS12 enables rapid recovery of redox potential (ΔE_GSH = -310 to -360 mV) post-H₂O₂ stress through:

  • Deglutathionylation of PRXIIE (k_cat = 4.7 × 10³ M^-1s^-1)

  • Recycling of 2-Cys peroxiredoxins via monothiol mechanism

Stress Response Modulation

• GRXS12-deficient plants exhibit:

  • Delayed roGFP2 reduction kinetics (t_1/2 increased by 38%) after oxidative challenge

  • Increased total glutathione levels (1.8-fold vs WT) under paraquat stress

Technical Considerations for GRXS12 Antibody Use

• Cross-reactivity: Shows specificity for GRXS12 isoforms over other class I GRXs (C1/C2 clade)

• Detection limits: Effective in plant protein extracts ≥1 μg total protein

• Buffer compatibility: Requires non-reducing conditions for glutathionylated target detection