Customize FMOGS-OX1 Antibody

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

FMOGS-OX1 antibody; FMO3 antibody; At1g65860 antibody; F12P19.2 antibody; Flavin-containing monooxygenase FMO GS-OX1 antibody; EC 1.14.13.237 antibody; Flavin-monooxygenase glucosinolate S-oxygenase 1 antibody; Putative flavin-containing monooxygenase 3 antibody

Target Names

FMOGS-OX1

Uniprot No.

Q9SS04

Target Background

Function

This antibody catalyzes the conversion of methylthioalkyl glucosinolates into methylsulfinylalkyl glucosinolates. It exhibits the ability to S-oxygenate both desulfo- and intact 4-methylthiobutyl glucosinolates, but demonstrates no activity with methionine, dihomomethionine or 5-methylthiopentaldoxime.

Database Links

KEGG: ath:AT1G65860

STRING: 3702.AT1G65860.1

UniGene: At.35830

Protein Families

FMO family

Tissue Specificity

Mainly expressed in leaves. Low levels in flowers and seeds.

Q&A

Basic Research Questions

How to validate FMOGS-OX1 antibody specificity for Arabidopsis thaliana studies?

Perform tripartite validation:

Western blot: Use knockout mutants (e.g., Arabidopsis T-DNA lines) to confirm absence of target band
ELISA: Compare binding affinity against recombinant FMOGS-OX1 vs. homologous proteins (FMOGS-OX2/OX3)
Immunohistochemistry: Verify subcellular localization matches predicted ER/Golgi patterns in root sections

What experimental controls are essential for FMOGS-OX1 co-immunoprecipitation assays?

Include:

Nuclease treatment: Add Ethidium Bromide/Benzonase to rule out DNA-mediated artifacts
Isotype controls: Use species-matched IgG for immunoprecipitation baseline
Competition assays: Pre-incubate antibody with excess antigen (≥10:1 molar ratio)

How to optimize FMOGS-OX1 detection in glucosinolate-rich tissues?

Address interference through:

Interference Source	Mitigation Strategy	Validation Method
Secondary metabolites	Methanol:acetone (7:3) fixation	LC-MS metabolite profiling
Protein crosslinking	0.1% SDS in extraction buffer	Coomassie total protein stain
Epitope masking	Antigen retrieval (10 mM citrate, pH 6.0)	Serial antibody dilution

Advanced Research Questions

How to resolve FMOGS-OX1 expression data contradictions between transcript and protein levels?

Implement orthogonal validation:

Transcript-Protein correlation
- Perform qRT-PCR (primers spanning exons 2-4)
- Parallel Western blot with chemiluminescent quantification
Post-translational analysis
- Phos-tag™ gels for phosphorylation status
- Cycloheximide chase experiments (t½ measurement)

What advanced techniques confirm FMOGS-OX1's role in substrate oxygenation?

Combine:

Isotope tracing: ¹⁴C-labeled dihomomethionine in mutant complementation lines
Enzymatic assays: Measure NADPH consumption at 340 nm (ε=6.22 mM⁻¹cm⁻¹)
Structural validation: Antibody-based crystallization chaperoning for FMO-GS complex

How to design CRISPR screens using FMOGS-OX1 antibody validation data?

Develop three-tier screening:

Tier 1

Guide RNAs targeting exons 5-7 (FAD-binding domain)

Tier 2

Antibody-based FACS sorting of KO populations

Tier 3

Specialized Methodologies

What proteomic strategies identify FMOGS-OX1 interaction partners in redox environments?

Employ:

Native PAGE-MS: 4-16% gradient gels with 2 mM DTT
PLA (Proximity Ligation Assay): Duolink® probes with ≤40 nm resolution
Crosslinking: DSS (Disuccinimidyl suberate) at 2 mM for 30 min

How to integrate FMOGS-OX1 data with multi-omics datasets?

Use systems biology pipelines:

Data Type	Alignment Strategy	Validation Metric
RNA-seq	Co-expression network analysis (WGCNA)	Pearson's r > 0.85
Metabolomics	Pathway enrichment (KEGG map00966)	Q-value < 0.01
ChIP-seq	Motif co-occurrence (HOMER analysis)	Positional p-value < 1e-5

What statistical approaches address FMOGS-OX1 antibody variability across developmental stages?

Implement mixed-effects models:

Key parameters:

Restricted Maximum Likelihood (REML) estimation
Kenward-Roger degrees of freedom

Technical Validation Frameworks

How to establish antibody performance metrics for multi-lab reproducibility?

Adopt MIATA guidelines with modifications:

Parameter	Acceptance Criteria	Measurement Method
Lot consistency	CV < 15% across 3 batches	ELISA EC₅₀ comparison
Cross-reactivity	≤5% binding to FMOGS-OX2	Surface plasmon resonance
Thermal stability	≥80% activity after 5 freeze-thaws	Functional assay

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FMOGS-OX1 Antibody