GSR Human

Glutathione Reductase Human Recombinant
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Description

GSR Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 504 amino acids (43-522) and having a molecular mass of 54.3kDa.
GSR is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Glutathione reductase (GSR) is an enzyme that helps maintain the balance of glutathione (GSH), a crucial antioxidant, within cells. It converts oxidized glutathione (GSSG) back to its reduced form (GSH), which is essential for protecting cells from damage caused by reactive oxygen species and supporting various cellular processes like protein and DNA synthesis.
Description
This product consists of the human GSR enzyme, produced in E. coli bacteria. It is a single chain of 504 amino acids with a molecular weight of 54.3kDa. The enzyme is engineered with a 24 amino acid His-tag at its N-terminus to facilitate purification.
Physical Appearance
Clear and colorless solution, sterilized by filtration.
Formulation
The GSR protein is supplied in a solution containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol, and 0.1M NaCl, with a protein concentration of 1mg/ml.
Stability
For short-term storage (up to 4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for extended storage. Avoid repeated freezing and thawing.
Purity
The purity is greater than 95% as determined by SDS-PAGE analysis.
Biological Activity
The specific activity of the enzyme is greater than 29 units per milliliter. One unit is defined as the amount of enzyme required to reduce 1.0 µmol of oxidized glutathione per minute at pH 7.5 and 25°C.
Synonyms
Glutathione reductase mitochondrial, GR, GRase, GSR, GLUR, GRD1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAMACRQ EPQPQGPPPA AGAVASYDYL VIGGGSGGLA SARRAAELGA RAAVVESHKL GGTCVNVGCV PKKVMWNTAV HSEFMHDHAD YGFPSCEGKF NWRVIKEKRD AYVSRLNAIY QNNLTKSHIE IIRGHAAFTS DPKPTIEVSG KKYTAPHILI
ATGGMPSTPH ESQIPGASLG ITSDGFFQLE ELPGRSVIVG AGYIAVEMAG ILSALGSKTS LMIRHDKVLR SFDSMISTNC TEELENAGVE VLKFSQVKEV KKTLSGLEVS MVTAVPGRLP VMTMIPDVDC LLWAIGRVPN TKDLSLNKLG IQTDDKGHII VDEFQNTNVK GIYAVGDVCG
KALLTPVAIA AGRKLAHRLF EYKEDSKLDY NNIPTVVFSH PPIGTVGLTE DEAIHKYGIE NVKTYSTSFT PMYHAVTKRK TKCVMKMVCA NKEEKVVGIH MQGLGCDEML QGFAVAVKMG ATKADFDNTV AIHPTSSEEL VTLR.

Q&A

What are the six ethical principles required for government social research with human participants?

Government Social Research requires adherence to six core ethical principles when conducting research with human participants:

  • Clear and defined public benefit

  • Sound application, conduct and interpretation

  • Data protection regulations

  • Specific and informed consent

  • Enabling participation

  • Minimizing personal and social harm

These principles must guide the entire research process from conception through dissemination, ensuring that studies serve legitimate public needs while protecting participants . Research conducted for government should aim to meet clearly defined, legitimate needs that inform government business and serve the public good, with emphasis on evidence for policy and decision-making .

How should researchers implement inclusive research practices in GSR studies?

Implementing inclusive research practices requires attention to multiple research phases:

Research Design Considerations:

  • Engage with diverse stakeholders early in planning

  • Review existing evidence for representation gaps

  • Select appropriate methodologies for capturing diverse experiences

  • Consider participatory approaches where appropriate

Data Collection Strategies:

  • Develop sampling frameworks that ensure representation of marginalized groups

  • Accommodate different accessibility needs in research materials

  • Provide multiple participation options (online, face-to-face, etc.)

  • Train researchers in cultural competence and sensitivity

When implementing these practices, researchers should align with the Public Sector Equality Duty (PSED), which requires public bodies to consider how policies or decisions affect people who are protected under the Equality Act . The GSR 2021-2025 strategy specifically prioritizes creating "a diverse and inclusive profession" and improving data generation about underrepresented groups whose views are less likely to be heard through traditional methods .

What methodological considerations are essential for sound application in GSR?

Methodological ComponentKey ConsiderationsEthical Implications
Study DesignAppropriateness for research questionsEnsures valid findings without unnecessary burden
SamplingRepresentativeness and inclusivityPrevents systematic exclusion of certain groups
Data CollectionMethod validity and participant burdenRespects participant time and wellbeing
AnalysisTransparency and rigorPrevents misrepresentation of findings
ReportingCompleteness and accessibilityEnsures public benefit is realized

Sound methodology in GSR requires researchers to select approaches that not only answer research questions effectively but also uphold ethical principles. This includes ensuring research is conducted by those with appropriate qualifications and skills, using methods suitable for capturing the experiences of diverse populations, and providing transparent reporting of limitations .

How does GSR measurement work and what can it tell us about human emotional states?

Galvanic Skin Response (GSR) measures the electrical conductance of skin, which changes in response to sympathetic nervous system activation during emotional arousal. The physiological mechanism involves:

  • Emotional stimuli activate the sympathetic nervous system

  • Eccrine sweat glands increase activity

  • Moisture on skin surface increases electrical conductance

  • Changes in conductance are measured via electrodes placed on skin

It's important to note that GSR signals reflect the intensity of emotional arousal rather than the valence (positive or negative nature) of emotions . Research has demonstrated connections between GSR and emotional arousal across multiple contexts, with applications in psychological research dating back over a century .

What are best practices for experimental design in GSR human research?

Rigorous GSR research requires careful attention to experimental design:

Stimulus Selection and Presentation:

  • Use validated stimuli with known emotional properties

  • Control for stimulus duration and intensity

  • Randomize or counterbalance presentation order

  • Include appropriate neutral baseline conditions

Participant Considerations:

  • Screen for medical conditions affecting sweating

  • Control for environmental factors (temperature, humidity)

  • Allow sufficient acclimation period before measurement

  • Minimize movement artifacts through proper instruction

Data Acquisition:

  • Place electrodes on sites with high eccrine sweat gland density (fingers, palms)

  • Maintain consistent electrode pressure and contact area

  • Use appropriate sampling rate (minimum 20Hz, preferably higher)

  • Apply standardized preprocessing steps (artifact removal, signal filtering)

Researchers should be aware that both positive ("happy" or "joyful") and negative ("threatening" or "saddening") stimuli can increase arousal and skin conductance, making GSR an indicator of emotional intensity rather than emotional type .

What advanced analytical approaches are recommended for GSR data interpretation?

Advanced GSR data analysis requires sophisticated approaches to extract meaningful information:

  • Signal Decomposition Techniques:

    • Continuous Decomposition Analysis (CDA) to separate tonic and phasic components

    • Deconvolution approaches to identify overlapping skin conductance responses (SCRs)

    • Non-negative deconvolution to maintain physiological plausibility

  • Feature Extraction Methods:

    • Amplitude metrics (peak height, area under curve)

    • Temporal metrics (rise time, recovery time, half-recovery time)

    • Frequency domain analysis (spectral power distribution)

  • Statistical Modeling Approaches:

    • Mixed effects models to account for individual differences

    • Time series analysis for temporal dependencies

    • Machine learning algorithms for pattern recognition

When interpreting results, researchers must consider individual baseline differences, habituation effects, and the potential influence of confounding variables like caffeine consumption, medication, and physical activity .

What are the standard collection methods for GSR samples from human subjects?

Collection of Gunshot Residue (GSR) from human subjects requires standardized approaches:

Adhesive Sampling Technique:

  • Use 1 × 1 cm strips of double-sided adhesive tape fixed against a thin acetate band

  • Stretch skin during collection to expose particles hidden within skin folds or hair follicles

  • Cover each sampled area at least three times, even if adhesive loses tackiness

  • Maintain consistency in coverage to ensure reliable interpretation

  • Particles can still adhere to the surface even when adhesive appears depleted

Sampling Locations:

  • Hands (highest concentration area for shooters)

  • Forearms

  • Clothing surfaces

The timing of collection is critical, as GSR particles can be lost through normal activities. Researchers should document time elapsed between the firearm discharge and sample collection .

What chemical tests are used for GSR identification and what are their limitations?

Several chemical tests are employed for GSR identification, each with specific applications and limitations:

Test NameTarget Elements/CompoundsVisual IndicatorLimitations
Sodium RhodizonateLeadRed/purple colorationNot specific to GSR only
Walker TestNitritesBlue colorationMany false positives
Greiss TestNitritesRed-orange spotsEnvironmental contaminants can interfere
Marshall TestNitratesBlue colorationLimited specificity
Di-Thio-Oxamide (DTO)CopperDark green/blackNot all ammunition contains copper
Harrison and GilroyLead, Barium, AntimonyColor changes for each elementRequires multiple reagents

These tests are generally considered presumptive rather than confirmatory. The Harrison and Gilroy reagent can detect multiple GSR components: orange coloration indicates antimony, red spots indicate barium or lead, and when sprayed with dilute HCl, purple spots confirm lead presence .

What advanced methods are available for non-invasive GSR detection on human subjects?

Recent advances have expanded options for non-invasive GSR detection:

Visual Examination Using Alternative Light Sources:

  • Light sources between 475-530 nm wavelength can visualize GSR particles

  • Fluorescent particles observed on shooter's hands under 520 nm appear similar to GSR particles seen on fabrics

  • Collection and analysis of these fluorescent particles have confirmed GSR presence

  • Infrared (IR) cameras show potential for GSR detection, though additional research is needed for particle composition verification

The primary advantage of this approach is the ability to detect GSR without destroying or removing particles prior to collection for confirmatory laboratory analysis. This preserves evidence integrity while providing rapid preliminary assessment .

When implementing these techniques, researchers must ensure proper documentation through photography and maintain strict chain of custody for any subsequently collected particles.

How should researchers approach informed consent for GSR studies with vulnerable populations?

Obtaining informed consent from vulnerable populations requires enhanced procedures:

  • Accessibility Considerations:

    • Provide information in multiple formats (written, verbal, visual)

    • Use clear, non-technical language appropriate to comprehension level

    • Allow sufficient time for information processing and questions

    • Consider involving trusted advocates when appropriate

  • Consent Documentation:

    • Develop tailored consent forms addressing specific vulnerabilities

    • Include clear explanations of participation risks and benefits

    • Describe data handling and confidentiality protections

    • Emphasize the voluntary nature of participation and right to withdraw

  • Ongoing Consent Process:

    • Implement continuous consent verification throughout the research

    • Monitor for signs of discomfort or reluctance

    • Reaffirm participation willingness at key research stages

    • Provide multiple opportunities to ask questions

The GSR ethical framework emphasizes that specific and informed consent must be obtained from all participants, with particular attention to those with limited capacity to consent or those in dependent relationships . This aligns with broader ethical requirements to ensure participation is genuinely voluntary and based on complete understanding.

How can researchers balance scientific rigor with minimizing harm in sensitive GSR research?

Balancing scientific rigor with harm minimization requires thoughtful research design:

Proactive Risk Assessment:

  • Conduct comprehensive risk analysis before study commencement

  • Consider physical, psychological, social, and reputational harms

  • Identify vulnerable participant subgroups requiring additional protections

  • Develop explicit mitigation strategies for identified risks

Research Design Modifications:

  • Adjust data collection methods to reduce participant burden

  • Implement stepped disclosure for sensitive topics

  • Use simulation or proxy measures when direct measurement poses significant risk

  • Consider mixed-methods approaches that combine less invasive measures with targeted intensive measurement

Post-Participation Support:

  • Provide thorough debriefing after participation

  • Offer referrals to appropriate support services

  • Implement follow-up procedures for participants experiencing adverse effects

  • Document and analyze all adverse events to improve future protocols

The ethical principle of minimizing personal and social harm requires researchers to assess both immediate and long-term consequences of participation, with special attention to potentially vulnerable groups . This includes considering how data collection methods might impact participants differently based on their personal circumstances.

What are the data protection requirements for GSR research involving sensitive human data?

Data protection in GSR research demands rigorous safeguards:

Regulatory Compliance:

  • Adhere to relevant data protection laws (e.g., GDPR, Data Protection Act)

  • Implement appropriate technical safeguards (encryption, access controls)

  • Limit data collection to necessary information (data minimization)

  • Establish clear data retention and destruction protocols

Special Category Data Considerations:

  • Apply enhanced protections for biometric data, health information, and demographic details

  • Obtain explicit consent for processing special category data

  • Document legal basis for processing under relevant exemptions

  • Implement additional security measures proportionate to sensitivity

Transparency Mechanisms:

  • Provide clear privacy notices explaining data uses

  • Document data sharing arrangements with explicit justification

  • Enable participant data access and correction where feasible

  • Maintain comprehensive data processing records

Government Social Research ethical principles specifically identify data protection regulations as a core principle, requiring researchers to handle personal data legally, securely, and transparently . This includes considering data protection from the earliest stages of research design through final data disposition.

What approaches are recommended for analyzing complex GSR data while maintaining participant protections?

Analysis of complex GSR data requires specialized approaches that maintain protections:

Anonymization and Pseudonymization:

  • Apply appropriate de-identification techniques before analysis

  • Create robust coding systems for linking related datasets

  • Consider statistical disclosure control methods for sensitive variables

  • Evaluate re-identification risks before data sharing or publication

Analytical Safeguards:

  • Develop analysis plans that minimize use of identifiable information

  • Aggregate data to appropriate levels to prevent individual identification

  • Apply statistical techniques that preserve privacy (differential privacy, synthetic data)

  • Document analytical decisions and their privacy implications

Reporting Considerations:

  • Review findings for inadvertent disclosure risks

  • Apply suppression rules for small cell sizes

  • Present findings at group rather than individual level

  • Balance transparency with confidentiality in methodology descriptions

Researchers must ensure sound application, conduct, and interpretation throughout the analytical process, preventing misuse or misinterpretation of findings, particularly when results might impact vulnerable groups .

How can GSR methodologies be integrated with other research approaches for comprehensive understanding?

Integrating GSR approaches with complementary methodologies enhances research depth:

Multi-method Integration Strategies:

  • Concurrent triangulation: simultaneously collecting quantitative and qualitative data

  • Sequential explanatory: using qualitative methods to explain quantitative findings

  • Mixed physiological approaches: combining GSR with other biometrics

  • Convergent validation: comparing findings across methodological approaches

Integration at Different Research Phases:

  • Design integration: developing protocols that incorporate multiple perspectives

  • Data collection integration: harmonizing measurement timing and approaches

  • Analysis integration: developing frameworks that synthesize diverse data types

  • Interpretation integration: creating explanatory models that draw on multiple data sources

Practical Implementation Approaches:

  • Establish common conceptual frameworks across methods

  • Develop integrated data management systems

  • Train research teams in multiple methodological approaches

  • Create analysis plans that explicitly address integration points

This integration approach supports the ethical principle of enabling participation by incorporating diverse research methods that can accommodate different participant preferences and needs .

What emerging technologies are changing GSR human research methodologies?

Emerging technologies are transforming GSR research approaches:

Government Social Research Innovations:

  • Digital ethnography tools for observing online social behavior

  • Remote consent platforms enhancing participation accessibility

  • Administrative data linkage improving sample representativeness

  • Natural language processing for analyzing qualitative data at scale

Galvanic Skin Response Advancements:

  • Wireless, wearable GSR sensors enabling ecological momentary assessment

  • Machine learning algorithms improving signal processing and artifact detection

  • Cloud-based analysis platforms supporting collaborative research

  • Virtual reality integration creating controlled yet immersive stimulus environments

Gunshot Residue Detection Developments:

  • Advanced imaging technologies improving non-invasive detection

  • Portable spectroscopic methods enabling rapid field analysis

  • Enhanced visualization techniques between 475-530 nm wavelengths

  • Infrared camera applications showing potential for GSR detection after image enhancement

When implementing these technologies, researchers must carefully evaluate ethical implications, including data security risks, potential for surveillance, and impacts on participant autonomy and dignity.

Product Science Overview

Introduction

Glutathione reductase (GR), also known as glutathione-disulfide reductase (GSR), is a crucial enzyme in cellular defense against oxidative stress. It catalyzes the reduction of glutathione disulfide (GSSG) to the sulfhydryl form glutathione (GSH), which is essential for maintaining the reducing environment of the cell .

Structure and Function

Glutathione reductase is a homodimeric flavoprotein disulfide oxidoreductase. Each monomer contains a flavin adenine dinucleotide (FAD) prosthetic group and utilizes NADPH as a reducing agent to convert GSSG into two molecules of GSH . This reaction is vital for the detoxification of reactive oxygen species (ROS) and maintaining cellular redox homeostasis .

Biological Significance

The enzyme plays a critical role in the prevention of oxidative damage within the cell. By maintaining high levels of reduced glutathione, GR helps protect cellular components from oxidative stress, which can lead to cell damage and death . This function is particularly important in tissues with high oxidative metabolism, such as the liver and red blood cells .

Recombinant Production

Recombinant human glutathione reductase is produced using various expression systems, including bacterial, yeast, and mammalian cells. The recombinant form is often used in research and therapeutic applications due to its high purity and activity . The production process involves cloning the human GSR gene into an expression vector, transforming the host cells, and purifying the expressed protein .

Clinical Relevance

Deficiency in glutathione reductase can lead to various health issues, including increased susceptibility to oxidative stress and hemolytic anemia . Monitoring GR activity is crucial in diagnosing and managing conditions related to oxidative stress .

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