GSTU9 Antibody

Overview of GST Antibodies

GST antibodies are immunological tools designed to detect or purify glutathione S-transferase (GST)-tagged proteins. GST is a 26 kDa enzyme from Schistosoma japonicum widely used in recombinant protein expression systems. Antibodies against GST enable applications such as:

• Western blotting

• Immunoprecipitation

• Immunofluorescence

• ELISA

Antibody Structure

• GST antibodies are typically monoclonal or polyclonal and recognize epitopes on the GST tag.

• Example: Mouse monoclonal anti-GST (Clone GST.B6) binds specifically to GST fusion proteins with high affinity .

GST Tag Function

• Facilitates protein purification via glutathione affinity chromatography.

• Enhances solubility of recombinant proteins .

Cancer Immunotherapy

• Anti-Galectin-9 (Gal-9) antibodies (e.g., Clone 292-4) block Gal-9-induced T-cell death, enhancing antitumor responses in pancreatic ductal adenocarcinoma and triple-negative breast cancer models .

• Efficacy demonstrated in synergizing with PD-1/CTLA-4 inhibitors .

Autoimmune Disease Biomarkers

• Anti-GST A1-1 antibodies are detected in 16% of autoimmune hepatitis patients and correlate with severe clinical outcomes .

Technical Performance

• THE™ GST Antibody (A00865) detects GST tags at N-terminal, internal, and C-terminal positions with equal sensitivity (Fig. 1, ).

• Proteintech’s GST Tag Antibody (66001-2-Ig) achieves WB detection at dilutions up to 1:50,000 .

Limitations and Future Directions

• No GSTU9-specific antibodies are reported in current literature or commercial catalogs.

• Cross-reactivity with endogenous GST isoforms remains a challenge in some applications .

• Neutralizing antibodies (e.g., anti-Gal-9) show promise in combinatorial cancer therapies but require further preclinical validation .