At2g44570 Antibody

Molecular Characterization of At2g44570

The At2g44570 gene encodes a putative glycosyl hydrolase (GH9), an enzyme class involved in the hydrolysis of glycosidic bonds in polysaccharides such as cellulose and hemicellulose. Key features include:

• Gene ID: AT2G44570 (Arabidopsis Genome Initiative)

• Protein Function: Associated with cell wall loosening and remodeling during plant growth .

• Expression Profile: Microarray data indicate downregulation under specific experimental conditions (e.g., in studies of auxin-regulated genes) .

Table 1: Key Functional and Regulatory Data for At2g44570

Cell Wall Metabolism Studies

The antibody enables detection of At2g44570 in plant tissues, aiding investigations into:

• Cellulose Biosynthesis: Glycosyl hydrolases modify cellulose microfibrils, influencing cell wall extensibility .

• Stress Responses: Downregulation of At2g44570 correlates with altered cell wall integrity under biotic/abiotic stress .

Genetic Regulation Analysis

Used in tandem with transcriptomic data, the antibody helps validate gene expression patterns. For example:

• Co-downregulation with expansins (e.g., EXP16) and cellulose synthases in auxin-related experiments .

Technical Validation and Limitations

• Specificity: Validated via Western blot and immunofluorescence in Arabidopsis extracts .

• Limitations: No cross-reactivity data for non-Arabidopsis species are available.

Significance in Plant Biology

The At2g44570 antibody fills a critical niche in studying plant developmental biology, particularly in:

• Crop Improvement: Engineering cell wall composition for biofuel production.

• Disease Resistance: Linking cell wall dynamics to pathogen defense mechanisms.