HAC5 Antibody

Introduction to HAC5 Antibody

The HAC5 Antibody is a mouse monoclonal antibody (IgG3 isotype) designed to specifically recognize the HA tag, a synthetic peptide derived from the hemagglutinin (HA) protein of human influenza virus (amino acids 98–106) . This epitope tag is widely used in molecular biology to facilitate detection, purification, and functional analysis of recombinant proteins. HAC5 binds to HA-tagged fusion proteins, regardless of their N-terminal, C-terminal, or internal positioning .

Structure and Specificity

Tested Applications

Validation Data

• Western Blot: HAC5 detects HA-tagged proteins in transfected HEK293 and 293 cell lysates (~85 kDa), with no signal in untransfected controls .

• ICC/IF: Clear nuclear and cytoplasmic staining observed in HA-tagged transcription factor-expressing cells, with DAPI counterstaining .

Research Relevance and Limitations

While HAC5 is not directly studied in the context of influenza immunity, its role in molecular biology aligns with broader HA antibody research. For example:

• Epitope Tagging: HA tags enable precise tracking of recombinant proteins, avoiding interference with endogenous antibodies .

• Cross-Reactivity: Unlike HA-specific antibodies targeting viral antigens (e.g., H5N1) , HAC5 is non-reactive to natural HA proteins, ensuring specificity in experimental systems .

Limitations:

• Species Reactivity: Primarily validated in human cell lines (HEK293, HeLa, 293) . Limited data for non-human systems.

• Conformational Dependence: Not explicitly tested for binding to denatured HA-tagged proteins, though WB compatibility suggests tolerance to SDS-PAGE conditions .

Comparative Analysis with Other HA Antibodies