HCN1 Antibody, Biotin conjugated
Description
Key Features of Biotin-Conjugated HCN1 Antibodies
Neurological and Cellular Studies
Biotin-conjugated HCN1 antibodies are critical for studying HCN1’s role in neuronal excitability and neurological disorders:
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Hippocampal Neuron Analysis: In mouse models of Hcn1 mutations (e.g., p.G391D, p.M153I), biotinylated secondary antibodies were used to detect reduced HCN1 protein levels in CA1 pyramidal neurons, correlating with diminished voltage sag and seizure susceptibility .
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Subcellular Localization: Antibodies targeting the C-terminal region (e.g., AA 778–910) confirmed HCN1’s presence in presynaptic terminals and dendritic compartments, enabling studies on its role in synaptic plasticity .
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Disease Modeling: Mutant HCN1-GD subunits exhibited impaired trafficking to axonal terminals, as shown via HA-tagged HCN1 and biotin-labeled detection systems .
Methodological Advantages
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High Specificity: The C-terminal epitope (AA 778–910) ensures minimal cross-reactivity, as validated in Western blot and IHC .
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Versatile Detection: Biotin conjugation allows integration with streptavidin-HRP or fluorescent probes, enhancing sensitivity in:
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ELISA Compatibility: Polyclonal variants enable quantitative analysis of HCN1 levels in serum or lysates .
Epitope Selection
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N-Terminal vs. C-Terminal: Antibodies targeting the N-terminus (AA 6–24) may detect denatured HCN1 in WB, while C-terminal antibodies (AA 778–910) are optimal for native protein detection in IHC .
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Species Reactivity: Human-specific polyclonal antibodies (e.g., A24483) lack cross-reactivity with rodent samples, requiring careful selection for interspecies studies .
Optimization Protocols
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Western Blot:
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Immunohistochemistry:
Comparative Analysis of Biotin-Conjugated Antibodies
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Polymorphism of HCN1 has been linked to breast cancer. PMID: 28178648
- A study utilizing cryo-electron microscopy has provided structures of the human HCN1 channel at 3.5 A resolution, both in the absence and presence of cAMP. These structures reveal that HCN channels possess a unique structure within their K(+) channel selectivity filter-forming sequence, explaining their permeability to both Na(+) and K(+). PMID: 28086084
- A novel mechanism for regulating HCN1 trafficking has been identified: a di-arginine ER retention signal that monitors the processing of the channel in the early secretory pathway. PMID: 25142030
- De novo point mutations in HCN1 have been found to cause a recognizable early-onset epileptic encephalopathy in humans. PMID: 24747641
- Using decoy peptides mimicking the FLNa-binding domain of HCN1, researchers have demonstrated that acute abrogation of the HCN1-FLNa interaction in neurons eliminates the punctate distribution of HCN1 channels in neuronal cell bodies. PMID: 24403084
- Studies suggest that HCN1 channels could be potential therapeutic targets for the treatment of depressive disorders. PMID: 23033536
- Following seizures, wild-type presynaptic HCN1 channel function experiences a persistent decrease. PMID: 23077068
- HCN1 channels play a significant role in maintaining spontaneous burst activity in embryonic cortical neuron cultures. PMID: 22094222
- In HCN1-deficient neurons of the ventral cochlear nucleus, hyperpolarization-activated currents are smaller and slower, input resistances are higher, and membrane time constants are longer compared to HCN1-expressing neurons. PMID: 21562186
- Genetic analysis of 48 Sudden unexpected death in epilepsy cases identified six novel and three previously reported nonsynonymous (amino acid changing) variants in HCN1, HCN2, HCN3 and HCN4. PMID: 21615589
- Increased cAMP levels in cells counteracted the up-regulation of HCN1 channels mediated by a TRIP8b construct specifically binding the CNBD. PMID: 21504900
- The amplitude of human HCN1 hyperpolarization activated current (Ih) rapidly increases after establishing the whole-cell configuration in HEK293 cells. PMID: 20806410
- Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20145138
- The helical secondary structure of the external S3-S4 linker of this pacemaker channel has been examined and found to influence its activation. PMID: 12668666
- HCN1 and HCN2 expression levels were measured using in situ hybridization and immunocytochemistry in hippocampi; the expression of HCN isoforms is dynamically regulated in both human and experimental hippocampal epilepsy. PMID: 12890777
- An amino acid substitution, A881T, was identified in HCN1 in one idiopathic generalized epilepsy patient. PMID: 17931874
- The polymorphism rs10941679 near HCN1/MRPS30 was also associated with percent dense area in breast cancer. PMID: 19232126
- Observational study of gene-disease association. (HuGE Navigator) PMID: 19232126
- Observational study of gene-disease association. (HuGE Navigator) PMID: 18454440
- Observational study of gene-disease association. (HuGE Navigator) PMID: 17931874
- Observational study of gene-disease association. (HuGE Navigator) PMID: 18081024
