HSFA6A Antibody
Description
Target Protein: HSFA6A Overview
HSFA6A belongs to the heat shock transcription factor (HSF) family and functions as a transcriptional activator in ABA-dependent stress signaling pathways . Key features include:
Subcellular Dynamics
Studies employing subcellular fractionation and transient expression assays demonstrated:
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Basal state: 60-70% cytoplasmic localization in untreated protoplasts
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Stress-induced nuclear translocation:
Genetic Interaction Studies
HSFA6A antibody facilitated the characterization of transgenic lines:
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Overexpression (OE) lines:
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Knockout mutants:
Key Applications in Experimental Systems
The HSFA6A antibody has been critical for:
Research Limitations and Future Directions
Current gaps addressed through antibody-based research:
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
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Q&A
FAQ: HSFA6A Antibody in Academic Research
Advanced Research Questions
How to resolve discrepancies in reported HSFA6A localization under abiotic stress?
Conflicting data may arise from:
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Stress-specific regulation: HSFA6A nuclear translocation varies with stress type (heat vs. drought) . Use subcellular fractionation + immunoblotting alongside confocal microscopy with GFP-tagged HSFA6A.
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Antibody cross-reactivity: Validate with tissue-specific HSFA6A overexpression lines and epitope-mapping assays (see Table 1).
Table 1: HSFA6A Antibody Cross-Reactivity Profile
How to design experiments investigating HSFA6A’s role in ABA-mediated thermotolerance?
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Genetic interaction studies: Combine HSFA6A mutants with ABA biosynthesis/signaling mutants (e.g., aba2, snrk2.6) under heat stress .
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Transcriptome profiling: Use RNA-seq to identify HSFA6A-dependent ABA-responsive genes (e.g., HSP17.6, RBOHD) under dual stress.
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Chromatin immunoprecipitation (ChIP): Verify direct binding of HSFA6A to promoters of ABA/stress-related genes using validated antibodies .
Data Contradiction Analysis
How to address inconsistent molecular weight observations for HSFA6A?
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Post-translational modifications: Phosphorylation at Ser/Thr residues may increase apparent MW. Treat samples with λ-phosphatase prior to Western blot .
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Alternative splicing: Check for splice variants using RT-PCR with primers spanning exon-exon junctions.
Methodological Recommendations
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Epitope characterization: Map antibody binding via truncation mutants of HSFA6A’s C-terminal domain .
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Multiplex assays: Pair Western blot with qRT-PCR to correlate protein levels with HSFA6A transcript dynamics under stress.
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Functional redundancy tests: Use double mutants (e.g., hsfa6a/hsfa6b) to unmask overlapping roles with paralogs .
