Customize HSFA7B Antibody

Description

HSFA7B Functional Overview

HSFA7B is a heat shock transcription factor involved in multiple stress adaptation pathways:

Function	Mechanism	Experimental Evidence
Salt Stress Tolerance	Binds E-box-like motifs to regulate ion homeostasis, ROS reduction, and osmotic adjustment	ChIP-seq/RNA-seq validation in Arabidopsis
Heat Stress Memory	Directly regulates ethylene signaling genes (e.g., EIN3, ETO1) at the shoot apical meristem	RNA-seq, mutant analysis
Cellulose Biosynthesis	Suppresses CESA and CSL genes to modulate cell wall dynamics under stress	Genetic silencing and overexpression assays
Transcriptional Memory	Forms heteromeric complexes (e.g., with HSFA2/HSFA3) to sustain stress memory	Co-IP/MS and mutant phenotyping

Potential Applications of an HSFA7B Antibody

While no studies explicitly using an HSFA7B antibody were identified, its hypothetical applications can be inferred from methodologies applied in HSFA7B research:

Protein Localization and Expression Profiling

Subcellular Localization: Confirm nuclear localization, as HSFA7B functions as a transcription factor .
Stress-Inducible Expression: Quantify protein levels under heat, salt, or ABA treatments using Western blotting .

Molecular Interaction Studies

Chromatin Immunoprecipitation (ChIP): Validate HSFA7B binding to target promoters (e.g., EIN3, ETO1) .
Protein Complex Analysis: Identify interaction partners like HSFA2/HSFA3 via co-immunoprecipitation (Co-IP) .

Mutant Phenotyping

Knockout/Overexpression Lines: Verify HSFA7B protein absence or accumulation in transgenic plants (e.g., hsfa7b mutants) .

Regulatory Targets of HSFA7B

HSFA7B directly modulates genes involved in:

Ethylene Signaling: EIN3, ETO1, SYD
Heat Shock Proteins (HSPs): HSP17.8, HSP18.2
Ion Transporters: Unspecified genes maintaining Na+/K+ balance

Phenotypic Consequences of HSFA7B Dysregulation

Condition	HSFA7B Mutant Phenotype	Wild-Type Rescue
Salt Stress	Reduced survival, ion imbalance, ROS accumulation	Overexpression improves tolerance
Heat Priming	Impaired thermomemory, SAM growth arrest	Complementation restores SAM activity
Cellulose Synthesis	Hyperactive CESA/CSL genes, reduced osmotic adjustment	Silencing restores normal growth

Research Gaps and Future Directions

Antibody Validation: No studies confirmed HSFA7B antibody specificity or cross-reactivity with paralogs (e.g., HSFA7A).
Cross-Species Reactivity: HSFA7B homologs in crops (e.g., tomato) may require species-specific antibody development .
Post-Translational Modifications: Phosphorylation or oligomerization states remain uncharacterized .

Product Specs

Buffer

Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

14-16 weeks (Made-to-order)

Synonyms

HSFA7B antibody; HSF10 antibody; At3g63350 antibody; F16M2.200 antibody; Heat stress transcription factor A-7b antibody; AtHsfA7b antibody; AtHsf-10 antibody

Target Names

HSFA7B

Uniprot No.

Q9M1V5

Target Background

Function

This antibody targets HSFA7B, a transcriptional activator that specifically binds to the DNA sequence 5'-AGAAnnTTCT-3', also known as heat shock promoter elements (HSE).

Database Links

KEGG: ath:AT3G63350

STRING: 3702.AT3G63350.1

UniGene: At.54047

Protein Families

HSF family, Class A subfamily

Subcellular Location

Cytoplasm. Nucleus.

Q&A

Heat Shock Factor A7b (HSFA7b) plays critical roles in thermomemory and stress response regulation, particularly in plant systems. Below are research-focused FAQs addressing key experimental and mechanistic considerations for working with HSFA7B antibodies in academic contexts:

Advanced Research Challenges

How to resolve contradictory data on HSFA7b’s thermomemory effects across tissues?

Issue: SAM-specific vs. leaf responses
Solution:
- Tissue-specific RNA extraction (laser microdissection of SAM)
- Compare HSP17.8 expression in SAM vs. leaves via RNA in situ hybridization
- Use pHSFA7b:GUS reporters to map spatial activity

What methodologies address HSFA7a/7b functional redundancy in mutant studies?

Approach:
- Phenotypic quantification: Compare survival rates of single/double mutants under triggering HS (e.g., 75% cell death in hsfa7a hsfa7b vs. 18% in WT )
- Transcriptomic profiling: Identify unique DEGs in double mutants (202 genes post-triggering HS )
- Protein interaction: Co-IP/MS to test heterodimerization

How to optimize antibody protocols for stress-timecourse experiments?

Critical parameters:
- Sampling time: 0.5 hr post-triggering HS for peak HSFA7b activity
- Fixation: Vacuum-infiltrated formaldehyde for ChIP in meristems
- Buffer: Include 20 mM β-mercaptoethanol to preserve redox-sensitive epitopes

Mechanistic & Technical Integration

What orthogonal methods confirm HSFA7b’s regulation of ethylene biosynthesis?

Combine:
- ChIP-seq: Identify direct targets (ETO1, EOL1 promoters )
- Metabolomics: Measure ACC (ethylene precursor) levels in hsfa7b mutants
- Pharmacology: Apply ethylene inhibitors (AVG) to rescue SAM defects

How to design studies linking HSFA7b to ABA cross-talk?

Strategy:
- Treat hsfa7b mutants with ABA and analyze HSP expression
- Use dual-luciferase reporters with HSFA7b and ABA-responsive promoters
- Profile Notabilis homologs in Arabidopsis for ABA-HSFA7b interactions

What multi-omics approaches contextualize HSFA7b network roles?

Layer	Technique	Application
Epigenomic	ATAC-seq	Identify accessible HSE regions post-priming
Proteomic	TurboID-MS	Map HSFA7b interactors under HS
Phenomic	ROS imaging	Quantify oxidative stress in mutant SAM

Data Interpretation Guidelines

Key metrics for thermomemory studies:
- SAM recovery rate (≥60% in WT vs. ≤30% in hsfa7b mutants )
- HSP17.8 induction fold-change (≥4x in primed SAM )
Troubleshooting low antibody signal:
- Validate heat-shock conditions (38°C for 90 min optimal )
- Check protein degradation (use protease inhibitor cocktails)

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HSFA7B Antibody