Customize HFI1 Antibody

Description

FLI1 Antibody

FLI1 is an ETS family transcription factor critical for regulating genes involved in hematopoiesis, vascular development, and oncogenesis .

HIF-1 alpha Antibody

HIF-1 alpha is a hypoxia-responsive transcription factor that regulates angiogenesis, metabolism, and tumor progression.

Research Applications of HIF-1 alpha Antibodies

Cancer Biology

HepG2 Hepatocellular Carcinoma: Western blot detection at 120 kDa under hypoxic conditions (1 mM DFO treatment) .
HeLa Cells: Nuclear localization confirmed via immunofluorescence .

Autoimmune & Inflammatory Diseases

HIF-1 alpha regulates CD55 in airway epithelium, influencing inflammatory responses .
MTA1/HIF-1 alpha axis exacerbates colitis via AQP4 upregulation .

Molecular Mechanisms

FLI1 Interaction: FLI1 modulates HIF-1 target gene specificity in endothelial cells under hypoxia .
LncRNA Regulation: LncRNA-SNHG6 promotes hepatocellular carcinoma via miR-6509-5p/HIF1A axis .

Common Quality Control Metrics

Method	Purpose	Example Data from Sources
Knockout Validation	Confirms target specificity	No signal in HIF-1 alpha KO HeLa cells
Cross-Reactivity	Species compatibility	Human/Mouse/Rat reactivity (AF1935)
Epitope Mapping	Antigen-binding region confirmation	C-terminal peptide immunization (39665)

Clinical Relevance

HIV Prevention: While unrelated to FLI1/HIF-1 alpha, the AMP Study (HVTN 703/HPTN 081) demonstrated antibody-mediated prevention using VRC01, a broadly neutralizing antibody against HIV .
Therapeutic Antibodies: Engineered antibodies targeting HIF-1 alpha are under investigation for cancer therapy .

Product Specs

Buffer

**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

HFI1 antibody; ADA1 antibody; SUP110 antibody; YPL254W antibody; Transcriptional coactivator HFI1/ADA1 antibody

Target Names

HFI1

Uniprot No.

Q12060

Target Background

Function

HFI1 Antibody functions as a component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different mechanisms such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. HFI1/ADA1 and SPT20/ADA5 may recruit TATA binding protein (TBP) and possibly other basal factors to bind to the TATA box.

Gene References Into Functions

Research indicates that the boundary function of Ada1 is functionally linked to proteasome processes. Furthermore, four relatively small regions within ADA1 contribute to boundary formation through distinct mechanisms. PMID: 27647735

Database Links

KEGG: sce:YPL254W

STRING: 4932.YPL254W

Subcellular Location

Nucleus.

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HFI1 Antibody