At3g24360 Antibody

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Description

Definition and Target Identification

The At3g24360 antibody is an immunoglobulin developed to detect and bind the protein product of the At3g24360 gene in Arabidopsis thaliana (mouse-ear cress). This gene identifier follows the standardized Arabidopsis genome nomenclature:

  • "At": Arabidopsis thaliana

  • "3g": Chromosome 3 genomic locus

  • "24360": Unique identifier for the gene locus .

The antibody’s specificity allows researchers to study the expression, localization, and interaction dynamics of the At3g24360 protein, which remains functionally uncharacterized in publicly available datasets.

Research Context and Applications

While direct studies using the At3g24360 antibody are not detailed in the reviewed literature, its existence implies utility in:

  • Protein Localization: Tracking spatial expression patterns in plant tissues (e.g., roots, leaves, or floral organs).

  • Gene Expression Analysis: Validating transcriptional activity under stress conditions or developmental stages.

  • Interaction Studies: Identifying binding partners via co-immunoprecipitation or yeast two-hybrid systems.

Antibodies against Arabidopsis proteins like At3g24360 are critical for dissecting metabolic pathways, stress responses, and developmental processes .

Comparative Analysis with Related Antibodies

The Cusabio catalog lists multiple antibodies targeting Arabidopsis proteins, revealing trends in plant research priorities. For example:

Target ProteinProduct CodeUniProt IDKey Research Focus
HIPP07CSB-PA548555XA01DOAQ9C5D3Heavy metal-associated proteins
HSFA4CCSB-PA884270XA01DOAQ9FK72Heat shock transcription factors
GSTU24CSB-PA879742XA01DOAQ9SHH6Glutathione S-transferases

This contextualizes At3g24360 within broader efforts to characterize Arabidopsis proteins involved in stress adaptation, metabolism, and signaling .

Challenges and Future Directions

The lack of published data on At3g24360 highlights gaps in functional genomics for non-model plant genes. Future research could:

  • Perform knockout/overexpression studies to elucidate its role.

  • Investigate post-translational modifications or subcellular localization.

  • Explore cross-species homology to infer potential functions.

Antibody validation through orthogonal methods (e.g., mass spectrometry or CRISPR-edited lines) would strengthen reliability .

Supplier Reliability and Usage Notes

Cusabio, the antibody provider, specializes in custom reagents for plant and animal research. Users should:

  • Validate batch-specific reactivity using positive/negative controls.

  • Optimize protocols for Arabidopsis-specific tissues, which often require specialized extraction buffers.

  • Cross-reference UniProt (Q9LK08) for updated annotations on At3g24360 .

Product Specs

Buffer
**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
At3g24360 antibody; K7M2.213-hydroxyisobutyryl-CoA hydrolase-like protein 4 antibody; mitochondrial antibody; EC 3.1.2.- antibody
Target Names
At3g24360
Uniprot No.

Target Background

Database Links

KEGG: ath:AT3G24360

STRING: 3702.AT3G24360.1

UniGene: At.50223

Protein Families
Enoyl-CoA hydratase/isomerase family
Subcellular Location
Mitochondrion.

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