HIF1A Antibody

What is HIF1A Antibody?

HIF1A antibodies are immunodetection reagents designed to bind specifically to the HIF1A protein, a subunit of the HIF-1 transcription factor. HIF1A is stabilized under hypoxic conditions and regulates genes involved in angiogenesis, metabolism, and cell survival . These antibodies are critical for identifying HIF1A expression levels in experimental models, particularly in cancer research and hypoxia-related pathologies.

Applications of HIF1A Antibodies

HIF1A antibodies are used across diverse experimental techniques, including:

Validation and Specificity

HIF1A antibodies undergo rigorous validation to ensure precision:

• Knockout Controls: Antibodies like MAB19351 and MAB1536 show no reactivity in HIF1A-knockout HeLa cells, confirming target specificity .

• Hypoxia Induction: HIF1A expression is upregulated in cells treated with hypoxia mimetics (e.g., CoCl₂, DFO) or low oxygen (1% O₂) .

• Cross-Reactivity: Some antibodies (e.g., MAB1536) detect HIF1A across species, including human, mouse, and rat .

Cancer Biology

• Tumor Angiogenesis: HIF1A overexpression correlates with VEGF upregulation and neovascularization in glioblastoma and breast cancer .

• Drug Resistance: HIF1A promotes chemoresistance by upregulating drug efflux pumps (MDR1, MRP1) and inhibiting apoptosis via Bcl-2/BNIP3 pathways .

• Prognostic Marker: Elevated HIF1A in early-stage breast cancer (e.g., ductal carcinoma in situ) predicts poor therapy response and metastasis .

Stroke and Ischemic Injury

• HIF1A stabilizes during cerebral hypoxia, driving adaptive responses (e.g., glycolysis, erythropoietin production) and pathological outcomes (e.g., neuronal death) .

Technical Considerations

• Post-Translational Modifications: HIF1A antibodies must distinguish between hydroxylated, acetylated, or phosphorylated forms .

• Buffer Conditions: Reducing environments (e.g., β-mercaptoethanol) improve detection of the 120-kDa band .