HINFP Antibody, Biotin conjugated
Description
Introduction to HINFP Antibody, Biotin Conjugated
The HINFP (Histone H4 Transcription Factor) Antibody, Biotin Conjugated is a specialized immunological reagent designed for detecting and analyzing the HINFP protein, a zinc-finger transcriptional regulator critical for histone H4 gene expression and chromatin organization . Biotin conjugation enables high-sensitivity detection through streptavidin-biotin interactions, facilitating applications like ELISA, Western blotting, and immunoprecipitation . This conjugate combines the specificity of anti-HINFP antibodies with the amplification capabilities of biotin-streptavidin systems, making it indispensable for epigenetic and transcriptional regulation studies .
Molecular Details
Primary Uses
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ELISA: Quantifies HINFP in human samples using streptavidin-HRP/AP systems .
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Western Blotting: Detects HINFP in lysates from liver tissues (human, mouse, rat) .
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Immunoprecipitation (IP): Isolates HINFP-protein complexes (e.g., MBD2 interactions) .
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Epigenetic Studies: Investigates HINFP’s role in repressing transposable elements (TEs) via Histone1 regulation .
Recommended Dilutions
Functional Insights
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Genome Stability: HINFP represses transposable elements (TEs) by maintaining Histone1 expression, preventing DNA damage (γH2Av markers) . Loss of HINFP correlates with TE activation and accelerated aging/cancer phenotypes .
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Biotinylation Role: Biotinylated histones (e.g., H4K12bio) repress TEs in humans, suggesting HINFP-biotin tools could study epigenetic silencing .
Technical Validation
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Specificity: The antibody detects a single band at 60–70 kDa in human, mouse, and rat tissues .
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Interference Considerations: Excess free biotin in samples may skew ELISA/WB results; filtration or dilution is advised .
Optimization Tips
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Biotin Interference: Endogenous biotin in egg yolk or serum samples requires blocking with streptavidin/biotin pre-treatment .
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Signal Amplification: Pair with streptavidin-HRP/AP for enhanced detection in low-abundance targets .
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Storage Stability: Aliquot to avoid repeated freeze-thaw cycles; glycerol (50%) prevents aggregation .
Limitations
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research has purified and functionally characterized HiNF-P, a critical transcription factor essential for E2F-independent activation of the histone H4 multigene family. PMID: 14585971
- Studies indicate that transcriptional repression of Rb by MIZF may play a crucial role in myogenic differentiation. PMID: 15541338
- HiNF-P directly interacts with p220(NPAT) to coactivate histone genes during S phase. PMID: 15988025
- Research suggests that transcriptional silencing of the 14-3-3sigma gene is caused by promoter CpG island methylation associated with MBD2, which may play a significant role in prostate cancer progression during the invasive and metastatic stages. PMID: 16786000
- Findings indicate that RFP acts as a mediator connecting several MBD proteins, facilitating the formation of a more potent transcriptional repressor complex. PMID: 17049487
- HiNF-P is a bifunctional regulator of cell cycle controlled histone H4 gene transcription. PMID: 17163457
- HiNF-P-dependent stabilization of p220NPAT reinforces signaling through the cyclin E/CDK2/p220NPAT pathway and contributes to the coordinated control of histone gene expression. PMID: 17176114
- HiNF-P/P220NPAT regulates the expression of nonhistone targets that influence cell cycle progression competency. PMID: 17974976
- A novel HiNF-P-specific conserved region has been identified as a DNA-binding determinant that mediates histone gene expression during the cell cycle. This discovery establishes HiNF-P as a cell cycle regulatory member of the zinc finger transcription factor family. PMID: 18850719
- Research suggests that cyclin-dependent kinase inhibitors selectively control the stimulation of the histone H4 gene promoter by the p220(NPAT)/HiNF-P complex. PMID: 19170105
