HIPP42 Antibody
Product Specs
Composition: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
KEGG: ath:AT3G04900
STRING: 3702.AT3G04900.1
Q&A
Here’s a structured FAQ collection for HIPP42 Antibody tailored to academic research scenarios, derived from technical specifications and research-use guidelines :
Advanced Research Questions
How can researchers confirm HIPP42 Antibody specificity in novel experimental systems?
-
Perform immunogen competition assays: Pre-incubate the antibody with recombinant HIPP42 protein (10–20x molar excess) to abolish target band signals in WB .
-
Validate using knockout/knockdown mutants of Arabidopsis thaliana.
What methodological adjustments resolve non-specific binding in immunoassays?
| Factor | Optimization Strategy |
|---|---|
| Blocking Buffer | Test BSA (3–5%) or casein-based blockers. |
| Washing Stringency | Increase Tween-20 concentration (0.1–0.5%) in TBST. |
| Antibody Dilution | Reduce concentration to 1:1000 and extend incubation time (overnight at 4°C). |
How to interpret discrepancies in HIPP42 detection across assays?
-
ELISA-WB mismatch: May indicate post-translational modifications (PTMs) affecting epitope accessibility. Perform deglycosylation or phosphatase treatments.
-
Quantitative variations: Normalize to housekeeping proteins and repeat under standardized lysate concentrations .
Technical Validation Table
Methodological Recommendations
How to optimize HIPP42 Antibody for low-abundance targets?
-
Use signal amplification systems (e.g., HRP-tyramide) in WB.
-
Combine with immunoprecipitation to concentrate target protein prior to detection.
