HIPP07 Antibody
Description
HIPP07 Protein Overview
HIPP07 (UniProt ID: Q9C5D3) is a member of the HIPP protein family in Arabidopsis thaliana, characterized by:
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Heavy Metal-Associated (HMA) domains: Two conserved CXXC motifs for metal ion coordination .
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Isoprenylation motif: A C-terminal prenylation site (CaaX box) for membrane anchoring .
Key functions:
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Binds divalent metals (Zn²⁺, Cu²⁺, Cd²⁺, Pb²⁺) via HMA domains .
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Facilitates metal detoxification and redistribution in plant tissues .
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Modulates cytokinin signaling by interacting with cytokinin oxidase/dehydrogenase (CKX) proteins .
Domain Architecture
| Domain/Feature | Description | Role |
|---|---|---|
| HMA domains | Two βαββαβ folds with CXXC motifs | Metal ion binding (Zn²⁺, Cu²⁺, Cd²⁺) |
| Prenylation site | C-terminal CaaX motif | PD (plasmodesmata) localization |
Metal-Binding Specificity
HIPP07 exhibits reversible binding to multiple metals, as demonstrated by:
Research Applications of HIPP07 Antibody
The HIPP07 antibody enables:
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Subcellular localization: Confocal microscopy confirms GFP-tagged HIPP07 localizes to plasmodesmata (PD) in Arabidopsis leaf epidermal cells .
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Protein-protein interaction studies: Co-immunoprecipitation identifies interactions with CKX proteins and viral movement proteins .
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Stress response analysis: Quantifying HIPP07 expression under metal toxicity (Cd²⁺, Zn²⁺) or abiotic stress (cold, drought) .
Table 1: HIPP07 Functional Studies
Notable Observations
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Target Background
Q&A
HIPP07 Antibody FAQs for Academic Researchers
HIPP07, a heavy metal transport/detoxification superfamily protein, serves as a critical marker for studying plant cell types, particularly in root stele and stress response pathways. Below are FAQs tailored to academic research scenarios, emphasizing experimental design, validation, and advanced analytical approaches.
Advanced Research Questions
How to resolve contradictions in HIPP07 localization data across studies?
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Analysis Framework:
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Technical Factors: Compare fixation methods (e.g., paraformaldehyde vs. cryopreservation), antibody dilution ratios, and imaging resolution .
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Biological Variants: Assess tissue-specific splice isoforms or post-translational modifications affecting epitope accessibility.
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Cross-Study Validation: Replicate experiments using publicly available datasets (e.g., single-nucleus RNA-seq clusters from peanut root cells) .
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Can HIPP07 antibody data be integrated with multi-omics datasets?
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Workflow:
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Spatial Transcriptomics: Correlate HIPP07 protein levels with HIPP07 mRNA expression in adjacent tissue sections.
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ATAC-Seq Integration: Overlay chromatin accessibility data (e.g., root stele ATAC peaks) to identify regulatory elements driving HIPP07 expression under stress .
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Table 1: Key omics integration tools:
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What experimental designs capture dynamic HIPP07 expression under stress?
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Approach:
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Time-Series Sampling: Collect tissue at multiple time points post-stress induction (e.g., heavy metal exposure).
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Dual Staining: Combine HIPP07 antibody with markers of oxidative stress (e.g., glutathione reductase).
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Quantitative Analysis: Use Halo™ or ImageJ to measure fluorescence intensity changes relative to controls .
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Data Management and Reproducibility
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Storage: Annotated images and analysis outputs (e.g., cell counts, intensity metrics) should be uploaded to institutional repositories with metadata detailing antibody batch, dilution, and imaging parameters .
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Reporting: Include raw data tables in supplements, specifying:
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Table 2: Example HIPP07 antibody dataset:
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| Sample | HIPP07+ Cells (%) | Mean Fluorescence Intensity | Condition |
|---|---|---|---|
| Control | 12.3 ± 1.2 | 450 ± 30 | Standard growth |
| Cd-Stress | 28.7 ± 2.1 | 920 ± 45 | 100 μM CdCl₂, 24h |
