HIST1H1B (Ab-66) Antibody

Introduction to HIST1H1B (Ab-66) Antibody

HIST1H1B (Ab-66) Antibody (PACO56632) is a polyclonal antibody specifically designed for research involving Histone Cluster 1 H1 Family Member B (Hist1H1B), a protein that plays a crucial role in chromatin structure and gene regulation. This antibody has been developed by raising it in rabbits against a specific peptide sequence around the site of lysine 66 derived from Human Histone H1.5 . The antibody demonstrates high reactivity with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.

The development of antibodies targeting histone proteins like HIST1H1B has significantly advanced our understanding of epigenetic regulation and chromatin dynamics. These antibodies serve as essential tools for researchers investigating the complex mechanisms of gene expression control and cellular differentiation. HIST1H1B (Ab-66) Antibody specifically enables the detection and analysis of Hist1H1B in different cell types, making it valuable for studies in epigenetics, cancer research, and developmental biology .

As a research tool, this antibody provides scientists with the ability to visualize and quantify the presence and distribution of HIST1H1B protein within cellular structures. This capability is particularly important given the protein's role in packaging DNA into chromatin and its subsequent impact on gene accessibility and expression patterns.

Antibody Characteristics

The HIST1H1B (Ab-66) Antibody presents a specific set of technical characteristics that define its experimental utility and applications. Understanding these specifications is essential for researchers to effectively incorporate this antibody into their experimental protocols and interpret results accurately.

The antibody is classified as a polyclonal antibody, which means it contains a heterogeneous mixture of antibodies that recognize different epitopes on the target antigen . This characteristic often provides robust detection capabilities across various experimental conditions. As a rabbit-derived antibody, it offers advantages in terms of yield and affinity that are typical of rabbit immunoglobulins.

The antibody has been specifically validated for enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) applications, with recommended dilution ranges optimized for each technique . These validated applications ensure reliable performance in detection and localization studies of the HIST1H1B protein in human samples.

Biological Role

HIST1H1B plays critical roles in chromatin organization and gene regulation, functioning as a key component in the packaging of DNA into chromatin structures. By binding to linker DNA between nucleosomes, HIST1H1B contributes to the formation of the macromolecular structure known as the chromatin fiber .

Beyond its structural role, HIST1H1B influences gene expression by affecting the accessibility of DNA to transcription factors and other regulatory proteins. Through its interaction with chromatin, HIST1H1B can either facilitate or inhibit gene expression, depending on the specific cellular context and the presence of other regulatory factors.

Research has also implicated HIST1H1B in muscle organ development, suggesting specialized functions beyond its general role in chromatin organization . This involvement in tissue-specific developmental processes highlights the complexity of histone protein functions beyond their structural contributions to chromatin.

The dysregulation of histone proteins like HIST1H1B has been linked to various diseases, including cancer and developmental disorders . These associations underscore the importance of proper histone function in maintaining cellular homeostasis and proper development.

Immunohistochemistry Applications

The HIST1H1B (Ab-66) Antibody has been specifically validated for immunohistochemistry (IHC) applications, making it a valuable tool for visualizing the distribution and localization of HIST1H1B protein in tissue samples. This application is particularly useful for studying the expression patterns of HIST1H1B in different cell types and tissues.

For IHC applications, the recommended dilution range is 1:20-1:200, which should be optimized depending on the specific tissue type and detection method being used . The antibody has demonstrated effective performance in paraffin-embedded human tissue samples, as exemplified by its successful application in human small intestine tissue at a dilution of 1:100 .

Immunohistochemical analysis using this antibody can reveal valuable information about the nuclear distribution of HIST1H1B, its association with chromatin structures, and potential alterations in expression or localization associated with disease states. This visual evidence complements molecular and biochemical data, providing spatial context for understanding HIST1H1B function.

ELISA Applications

Enzyme-linked immunosorbent assay (ELISA) represents another validated application for the HIST1H1B (Ab-66) Antibody. This technique allows for the quantitative detection of HIST1H1B protein in various sample types, providing researchers with a means to measure protein levels with high sensitivity.

For ELISA applications, the recommended dilution range is 1:2000-1:10000, which should be optimized based on the specific ELISA protocol and detection system being employed . This wide dilution range suggests high sensitivity of the antibody when used in ELISA formats.

ELISA-based detection of HIST1H1B can be particularly valuable for studies investigating changes in histone protein expression under different experimental conditions or disease states. The quantitative nature of ELISA provides objective measurements that can be statistically analyzed to identify significant changes or correlations.

Other Experimental Techniques

While the HIST1H1B (Ab-66) Antibody has been specifically validated for ELISA and IHC, similar antibodies targeting HIST1H1B have demonstrated utility in additional experimental techniques that researchers might consider exploring. These applications expand the potential research contexts in which HIST1H1B can be studied.

Western blot analysis represents a common application for HIST1H1B antibodies, enabling the detection of the protein in cell or tissue lysates based on molecular weight separation . This technique can provide information about protein size, potential post-translational modifications, and relative abundance across different samples.

Immunofluorescence (IF) is another technique commonly employed with HIST1H1B antibodies, allowing for high-resolution visualization of protein localization within cells . This approach can be particularly valuable for colocalization studies with other nuclear proteins or chromatin markers.

Additional techniques that have been utilized with HIST1H1B antibodies include flow cytometry (FCM) for analyzing protein expression in individual cells within a population, and chromatin immunoprecipitation (ChIP) for studying the genomic regions associated with HIST1H1B binding . These diverse applications demonstrate the versatility of HIST1H1B antibodies in epigenetic and chromatin research.

A Comparative Analysis with Other HIST1H1B Antibodies

The HIST1H1B (Ab-66) Antibody represents one of several commercially available antibodies targeting the HIST1H1B protein. Understanding how this specific antibody compares with others in terms of epitope recognition, host species, applications, and performance characteristics can help researchers select the most appropriate tool for their specific research questions.

The antibody market offers a diverse range of HIST1H1B antibodies with varying specifications. These include antibodies targeting different epitopes of the protein, such as N-terminal regions, specific phosphorylation sites (e.g., S188, T17), and acetylation sites (e.g., K16) . Each of these targeting strategies provides different insights into HIST1H1B biology, with post-translational modification-specific antibodies being particularly valuable for studying regulatory mechanisms.

In addition to epitope differences, HIST1H1B antibodies vary in terms of host species (rabbit, mouse, etc.), clonality (monoclonal vs. polyclonal), and conjugation status (unconjugated vs. conjugated to fluorophores, enzymes, or other labels) . These variations influence the applications for which each antibody is best suited and their compatibility with other reagents in multiplexed experiments.

The HIST1H1B (Ab-66) Antibody specifically targets the region around lysine 66 of the protein , which distinguishes it from other commercially available antibodies that target different regions or modifications. This specificity may provide advantages for certain research questions, particularly those focused on this region of the protein or where other epitopes might be masked by protein interactions or modifications.

This comparative analysis demonstrates that while the HIST1H1B (Ab-66) Antibody offers specific advantages for certain applications, researchers should consider the full range of available antibodies when designing experiments to study HIST1H1B function and regulation.

Cancer Research

The study of histone proteins, including HIST1H1B, has become increasingly relevant in cancer research due to the critical role these proteins play in regulating gene expression. Dysregulation of histone proteins has been linked to various cancer types, making antibodies like HIST1H1B (Ab-66) important tools for investigating these connections.

Researchers can employ the HIST1H1B (Ab-66) Antibody to examine alterations in HIST1H1B expression, localization, or post-translational modifications in cancer cells compared to normal tissues . Such investigations may reveal patterns associated with specific cancer types or stages, potentially identifying HIST1H1B as a biomarker or therapeutic target.

The antibody's validated use in immunohistochemistry makes it particularly valuable for analyzing HIST1H1B expression patterns in human cancer tissue samples. These analyses can help establish correlations between HIST1H1B expression and clinical parameters such as tumor grade, patient prognosis, or response to specific therapies.

Furthermore, by targeting chromatin remodeling processes, researchers can use this antibody to explore the potential of developing therapeutics that modulate HIST1H1B function or expression . Such approaches could represent novel strategies for cancer treatment, particularly for cancers associated with epigenetic dysregulation.

Developmental Disorders

Beyond cancer research, HIST1H1B has been implicated in developmental processes and associated disorders. The HIST1H1B (Ab-66) Antibody provides researchers with a means to investigate these connections through detailed analysis of HIST1H1B expression and function during development.

HIST1H1B plays a role in muscle organ development, suggesting its importance in tissue-specific developmental programs . Researchers can utilize the antibody to examine HIST1H1B expression patterns across developmental stages and in various tissue types, potentially elucidating its role in normal development and developmental disorders.

Developmental disorders associated with chromatin regulation may involve alterations in histone proteins like HIST1H1B . The antibody enables researchers to compare HIST1H1B expression and localization between normal and affected tissues, potentially identifying mechanisms contributing to developmental abnormalities.

These investigations could ultimately lead to improved understanding of developmental disorders with epigenetic components and potentially inform therapeutic strategies targeting chromatin regulation mechanisms.