HIST1H2AG (Ab-29) Antibody

Introduction to HIST1H2AG (Ab-29) Antibody

The HIST1H2AG (Ab-29) Antibody is a polyclonal antibody developed against a peptide sequence surrounding residue Arg29 of human histone H2A type 1 (UniProt ID: P0C0S8). It is widely used to study epigenetic modifications, chromatin dynamics, and B-cell-mediated immune responses . This antibody targets the mono- or di-methylated Arg29 post-translational modification site, making it critical for investigating histone-associated transcriptional regulation .

Epigenetic Regulation

• Detects H2AK119ub (ubiquitinated histone H2A), a repressive chromatin mark linked to transcriptional silencing and DNA repair .

• Validated in models studying B-cell intrinsic regulation of antibody-mediated immunity, where BAP1 deficiency alters H2AK119ub levels and disrupts plasma cell differentiation .

Immunological Studies

• Used to analyze germinal center B-cell proliferation and class-switch recombination defects in Bap1 knockout murine models .

• Critical for mapping histone modifications in activated B cells during humoral immune responses .

Disease Research

• Applied in glioma tissue analysis (IHC) and HeLa cell immunofluorescence to study histone dynamics in cancer .

• Detects aberrant H2A ubiquitination in myelodysplastic syndrome and T-cell dysfunction .

Role in B-Cell Immunity

• BAP1 (BRCA1-associated protein 1) regulates genome-wide H2AK119ub levels, which are essential for B-cell proliferation and antibody production .

• Loss of BAP1 in B cells reduces plasma cell numbers by 90% and impairs antigen-specific IgG1 responses .

Histone Ubiquitination Mechanics

• 2A-HUB, an E3 ligase, specifically monoubiquitinates H2A at K119, repressing transcription by blocking RNA polymerase II release .

• Overexpression of 2A-HUB increases H2AK119ub levels, while its inactive mutant (2A-HUBR) has no effect .

Validation Data

• Western Blot: Detects a ~15 kDa band in 293 whole-cell lysates (observed at 32 kDa due to post-translational modifications) .

• Immunofluorescence: Localizes to nuclei in HeLa cells .

• IHC-P: Strong nuclear staining in human glioma tissues .