HNRNPAB Antibody, Biotin conjugated

Key Properties

The comprehensive specifications above represent standardized parameters for the biotin-conjugated HNRNPAB antibody products available from various suppliers .

Target Protein: HNRNPAB

Understanding the target protein is essential for proper application of the HNRNPAB antibody. HNRNPAB (Heterogeneous Nuclear Ribonucleoprotein A/B) belongs to the hnRNP protein family, a group of RNA-binding proteins crucial for RNA processing.

Molecular Function

HNRNPAB functions primarily as an RNA-binding protein with specific preferences for certain RNA sequences. Key functional characteristics include:

• Binds single-stranded RNA with high affinity

• Shows preference for G-rich and U-rich regions of heterogeneous nuclear RNA (hnRNA)

• Binds to APOB mRNA transcripts specifically around the RNA editing site

• Participates in RNA metabolism and processing pathways

These molecular functions contribute to HNRNPAB's important role in post-transcriptional regulation of gene expression.

Alternative Names and Identification

HNRNPAB is known by several alternative names in scientific literature, which is important to recognize when conducting research:

• ABBP-1 (APOBEC1-binding protein 1)

• ABBP1

• Apolipoprotein B mRNA editing enzyme catalytic polypeptide 1-protein 1

• hnRNP A/B

• HNRPAB

This diversity of nomenclature reflects both the protein's discovery history and its various functional associations.

Potential Research Areas

Based on the biological significance of HNRNPAB, this antibody can be valuable in several research domains:

• RNA processing and metabolism studies

• Post-transcriptional regulation investigations

• Cancer research, particularly regarding epithelial-mesenchymal transition

• Hepatocellular carcinoma research

• Investigations of RNA-protein interactions

The biotin conjugation provides specific advantages for detection sensitivity, making it particularly useful in applications where signal amplification is needed.

HNRNPAB in Disease Mechanisms

Research using antibodies against HNRNPAB has revealed significant roles of this protein in disease processes, particularly cancer. These findings enhance the value of HNRNPAB antibodies as research tools.

Role in Cancer Progression

Significant research has demonstrated that HNRNPAB contributes to tumor growth and metastasis, particularly in hepatocellular carcinoma (HCC) . Key findings include:

• HNRNPAB is overexpressed in highly metastatic cells

• It is elevated in tumor tissues from HCC patients with recurrence

• HNRNPAB overexpression promotes epithelial-mesenchymal transition (EMT)

• This promotion of EMT is associated with increased HCC metastasis both in vitro and in vivo

Molecular Mechanisms

At the molecular level, HNRNPAB has been shown to:

These findings position HNRNPAB as a potential biomarker and therapeutic target, underscoring the importance of reliable antibodies like the biotin-conjugated variant for studying this protein.

Technical Considerations for Antibody Use

When using HNRNPAB Antibody, Biotin conjugated in research applications, several technical considerations should be addressed to optimize results:

Detection Systems

As a biotin-conjugated antibody, this reagent is designed to work with streptavidin-based detection systems, which provide several advantages:

• High affinity between biotin and streptavidin allows for strong, specific signal detection

• Signal amplification is possible through various streptavidin-conjugated reporter molecules

• Compatible with multiple detection modalities (colorimetric, fluorescent, chemiluminescent)

Comparison with Other HNRNPAB Antibodies

The biotin-conjugated HNRNPAB antibody represents one of several formats available for detecting this protein. Other variants include:

Comparative Analysis Table

This diversity of antibody formats allows researchers to select the most appropriate tool for their specific experimental requirements.