HNRNPC Antibody
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Description
Definition and Development of HNRNPC Antibodies
HNRNPC antibodies are monoclonal or polyclonal reagents that specifically bind to HNRNPC proteins (C1/C2 isoforms), which regulate RNA splicing, stabilization, and translation. Key development methods include:
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Hybridoma Technology: Immunization with HNRNPC-derived peptides followed by B-cell fusion with myeloma cells (e.g., Clone 9G1 ).
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Recombinant Production: Cloning variable regions of antibody heavy/light chains into expression vectors for large-scale purification .
Validation involves rigorous testing across six applications (ELISA, WB, IHC, IF, FC, IP) to confirm specificity for human HNRNPC .
Cancer Biomarker Validation
HNRNPC antibodies have identified overexpression in 15+ cancers, including:
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Liver Cancer: Positive HNRNPC expression correlates with tumor size, microvascular invasion, and reduced survival (HR = 1.8, p < 0.01) .
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Prostate Cancer: Elevated HNRNPC links to higher Gleason scores (OR = 2.4) and suppressed immune infiltration (CD8+ T-cell reduction: 35%) .
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Cervical Cancer: HNRNPC promotes lymph node metastasis (p < 0.001) via FOXM1 splicing .
Functional Studies
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Proliferation Assays: HNRNPC knockdown reduces HCC cell viability by 60% (CCK-8 assay) .
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Invasion/Migration: Transwell assays show 70% inhibition in HNRNPC-silenced cervical cancer cells .
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Immune Microenvironment: IHC reveals HNRNPC negatively correlates with ImmuneScore in 23/25 cancers .
Clinical Implications
Technical Considerations
Product Specs
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. Stored at -20°C. Avoid repeated freeze-thaw cycles.
Lead Time
Typically, we can ship products within 1-3 business days after receiving your order. Delivery times may vary depending on the purchasing method or location. Please consult your local distributors for specific delivery details.
Synonyms
C1 antibody; C2 antibody; Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody; Heterogeneous nuclear ribonucleoprotein C antibody; Heterogeneous nuclear ribonucleoproteins C1/C2 antibody; HNRNP antibody; hnRNP C1 / hnRNP C2 antibody; hnRNP C1/C2 antibody; Hnrnpc antibody; HNRPC antibody; HNRPC_HUMAN antibody; MGC104306 antibody; MGC105117 antibody; MGC117353 antibody; MGC131677 antibody; Nuclear ribonucleoprotein particle C1 protein antibody; Nuclear ribonucleoprotein particle C2 protein antibody; SNRPC antibody
Target Names
HNRNPC
Uniprot No.
Target Background
Function
HNRNPC antibody binds to pre-mRNA and initiates the formation of 40S hnRNP particles. It interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA, influencing the stability and translation efficiency of the bound mRNA molecules. Single HNRNPC tetramers bind to 230-240 nucleotides, while trimers of HNRNPC tetramers bind to 700 nucleotides. HNRNPC may play a crucial role in the initial stages of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) through a mechanism known as the 'm(6)A-switch.' This modification facilitates binding of HNRNPC, ultimately regulating mRNA splicing.
Gene References Into Functions
References:
- APP levels decline progressively with age, correlating with the gradual normalization of FMRP and hnRNP C levels. PMID: 26048669
- Endoplasmic reticulum resident chaperone GRP78, mitochondrial protein Prohibitin, and heterogeneous nuclear ribonucleoprotein hnRNPC (C1/C2) interact with viral RNA. These proteins are considered key players in endoplasmic reticulum stress-induced apoptosis during JEV infection. PMID: 28102850
- Our findings demonstrate that a balance between lncRNA SNHG1 and hnRNPC regulates p53 activity and p53-dependent apoptosis in response to doxorubicin treatment. Furthermore, these findings suggest that changes in lncRNA subcellular localization under specific circumstances have biological significance. PMID: 28264987
- Our data suggest an interplay between CELF2 and hnRNP C as the underlying mechanism for activation-dependent alternative splicing of TRAF3 exon 8. PMID: 28031331
- Our data indicate that hnRNPC1/C2 binds to both DNA and RNA, influencing both gene expression and RNA splicing. However, these actions do not appear to be linked through 1,25(OH)2D-mediated induction of transcription. PMID: 27672039
- These findings suggest that hnRNP C1/C2 participates in dengue virus replication during the stage of viral RNA synthesis. PMID: 25890165
- HnRNP C, YB-1, and hnRNP L work together to enhance skipping of human MUSK exon 10, generating a Wnt-insensitive MuSK isoform. PMID: 25354590
- Our results suggest that hnRNP C1 controls HPV16 late gene expression. PMID: 25878250
- m(6)A-switch-regulated HNRNPC-binding activities affect the abundance and alternative splicing of target mRNAs, highlighting the regulatory role of m(6)A-switches in gene expression and RNA maturation. PMID: 25719671
- PTBP1 and hnRNP C repress exon 3 inclusion, and downregulation of PTBP1 inhibits BIM-mediated apoptosis. PMID: 24743263
- Downregulation of MALAT-1 expression impairs the cytoplasmic translocation of hnRNP C during the G2/M phase, resulting in G2/M arrest. PMID: 23973260
- Heterogeneous nuclear ribonucleoprotein C is a key regulator of BRCA gene expression and homologous recombination-based DNA repair. PMID: 23585894
- By preventing U2AF65 binding to Alu elements, hnRNP C serves as a genome-wide sentinel, safeguarding the transcriptome. PMID: 23374342
- Our data indicate that hnRNPC controls the aggressiveness of glioblastoma cells through the regulation of PDCD4. Silencing of hnRNPC lowers miR-21 levels, subsequently increasing the expression of PDCD4 and suppressing Akt and p70S6K activation. PMID: 22907752
- p27kip1, upregulated by hnRNPC1/2, antagonizes CagA-mediated pathogenesis. PMID: 22404445
- This study elucidates the mechanism by which Pol II transcripts are classified based on length. A heterotetramer of hnRNP C1/C2 acts as a molecular ruler, binding to unstructured RNA regions longer than 200 to 300 nucleotides. PMID: 22461616
- One of the identified proteins, hnRNPC, interacts with small hepatitis delta virus antigen in vitro and in vivo in human liver cells. PMID: 21774814
- Studies have shown that DDX21, HNRNPC, and RCC2 are isolated from the Ku86 multicomponent complex in response to DNA damage. PMID: 20873769
- HNRNP C recognizes uridine tracts with a defined long-range spacing, consistent with HNRNP particle organization. PMID: 20601959
- FMRP represses translation by recruiting APP mRNA to processing bodies, while hnRNP C promotes APP translation by displacing FMRP, thereby relieving the translational block. PMID: 20473314
- The 5' end of poliovirus negative-strand RNA interacts with endogenous hnRNP C, as well as with poliovirus nonstructural proteins. PMID: 20164237
- It is proposed that hnRNP C interacts with poliovirus RNA and replication proteins to enhance the efficiency of viral genomic RNA synthesis. PMID: 20189623
- Phosphorylation of heterogeneous nuclear ribonucleoprotein C1/C2 occurs in response to physiological levels of hydrogen peroxide in endothelial cells. PMID: 11877401
- Nuclear ribonucleoproteins C1 and C2 are part of the RNP complex that forms on XIAP IRES. The cellular levels of hnRNPC1 and -C2 parallel the activity of XIAP IRES, and overexpression of hnRNPC1-C2 specifically enhances translation of XIAP IRES. PMID: 12482981
- hnRNP C, through internal ribosomal entry site binding, modulates translation of c-myc mRNA in a cell cycle phase-dependent manner. PMID: 12509468
- The acidic C-terminal domain of hnRNP-C1/C2 may be a regulatory domain and play a critical role in the regulation of mRNA binding by hnRNP-C1/C2. PMID: 12564933
- A novel nuclear export pathway activated by the ROCK signaling pathway excludes hnRNP C1/C2 from the nucleus. This disruption of compartmentalization of specific hnRNP components occurs in apoptotic cells. PMID: 15494373
- CK1alpha-mediated phosphorylation modulates the mRNA binding ability of hnRNP-C. PMID: 15687492
- These findings suggest that hnRNP C1 plays a role in positive-strand RNA synthesis in poliovirus-infected cells, potentially at the level of initiation. PMID: 15731220
- An upstream element in human papillomavirus type 16 interacts specifically with CstF-64, hnRNP C1/C2, and polypyrimidine tract binding protein, suggesting that these factors enhance or regulate polyadenylation at the HPV-16 early polyadenylation signal. PMID: 15767428
- Direct in vivo interaction of hnRNPC with the urokinase receptor mRNA 3'UTR has been demonstrated. PMID: 16010978
- This study describes the purification of a locus control region-associated remodeling complex consisting of heterogeneous nuclear ribonucleoprotein C1/C2, SWI/SNF, and MeCP1 as a single homogeneous complex. PMID: 16217013
- Our results demonstrate that hnRNP C1/C2 is involved in maintaining cellular homeostasis, in addition to cellular differentiation and proliferation. PMID: 16960656
- Beyond its RNA-processing functions, hnRNP C1/C2 may be a key determinant of the temporal patterns of VDRE occupancy. PMID: 17071612
- The regulation of IRES-mediated translation by hnRNP C1/C2 and Unr might be significant during mitosis. PMID: 17159903
- A novel cis-element in the 5' coding region of p53 mRNA and its interaction with heterogeneous nuclear ribonucleoprotein (hnRNP)C1/C2 are described. PMID: 18296503
- Intracellular NS1 protein of dengue virus interacts with hnRNP C1/C2. PMID: 18471994
- The RNA binding domain of heterogeneous nuclear ribonuclear protein C binds and stabilizes urokinase-type plasminogen activator receptor (uPAR) mRNA, attributing the key role of this domain in hnRNPC-mediated uPAR regulation in lung epithelial cells. PMID: 18494499
- The H(2)O(2) responsive pre-mRNA binding protein hnRNP-C is upregulated in atherosclerosis. PMID: 18508286
- Anti-hnRNP C1/C2 antibody hinders splicing of SMN1 exon 7, but does not affect splicing of SMN2 exon 7. PMID: 19628962
- Endogenous hnRNP C and PTEN interact and co-localize within the nucleus. PMID: 19740742
- This protein has been found differentially expressed in Wernicke's Area from patients with schizophrenia. PMID: 19405953
Database Links
Protein Families
RRM HNRPC family, RALY subfamily
Subcellular Location
Nucleus. Note=Component of ribonucleosomes.
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