HOS2 Antibody
Description
Hos2 Antibody in Fungal Systems
Hos2 is a class I histone deacetylase (HDAC) critical for fungal virulence and gene regulation. Studies in Ustilago maydis and Saccharomyces cerevisiae demonstrate its role in epigenetic modulation and pathogenicity.
Key Research Findings:
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Role in Pathogenesis:
Hos2 deletion mutants in U. maydis show a 3-fold reduction in tumor formation and 6-fold decrease in plant death during infection, indicating its necessity for virulence . -
Gene Activation:
In S. cerevisiae, Hos2 binds coding regions of highly active genes during transcription, deacetylating H3 and H4 tails to promote efficient RNA polymerase II activity .
Experimental Antibody Use:
While no commercial Hos2 antibodies are detailed in the provided sources, research employs tagged constructs (e.g., Hos2-GFP or Myc-tagged Hos2) for localization and functional studies .
HO-2/HMOX2 Antibody in Mammalian Systems
HO-2 (HMOX2) is a heme oxygenase involved in heme catabolism. The Human/Mouse HO-2/HMOX2 Antibody (MAB3170) is a commercially available monoclonal antibody with validated applications.
Functional Validation:
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Western Blot: Detects HO-2 in human hepatocellular carcinoma (HepG2) and lung carcinoma (A549) cells, with no cross-reactivity to HO-1 .
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Hypersensitivity Assays: Functional Hos2-GFP fusion proteins rescue hos2Δ mutants in fungal models, confirming antibody utility in tracking protein localization .
Comparative Analysis of HOS2 and HO-2 Antibodies
| Feature | Hos2 (Fungal HDAC) | HO-2 (Mammalian HMOX2) |
|---|---|---|
| Biological Role | Epigenetic regulation, virulence | Heme degradation, antioxidant defense |
| Antibody Type | Research-grade (tag-dependent) | Commercial (MAB3170) |
| Key Applications | Localization, gene expression studies | Disease research, diagnostic assays |
Research Implications
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research has shown that histone deacetylases (Rpd3 and Hos2) play an unexpected role in promoting Spt6 interaction with elongating Pol II. PMID: 25182531
- Studies have observed the formation of a reversible cytosolic "stationary-phase granule" by Hos2, a nuclear histone deacetylase. In the stationary phase, hos2 mutants exhibit reduced viability. PMID: 22337769
- Both Elp3-induced acetylation and Hos2-induced deacetylation influence the nucleosome positions in the promoters of 24 genes. PMID: 21297980
- Hos2 and Set3 function at tRNA genes to promote Ty1 integration. PMID: 16415356
- Two histone deacetylases, RPD3 and HOS2, are essential for the activation of DNA damage-inducible genes RNR3 and HUG1. PMID: 17296735
- The Hos2p/Set3p complex (SET3C) is a novel component crucial for cell survival in response to secretory stress. PMID: 18487345
KEGG: sce:YGL194C
STRING: 4932.YGL194C
