HOXA11 Antibody, FITC conjugated
Description
Introduction to HOXA11 Antibody
HOXA11 belongs to the AbdB homeobox gene family and regulates anterior-posterior patterning during embryogenesis. It is essential for uterine development, female fertility, and the cyclic remodeling of the endometrium . In cancer, HOXA11 overexpression promotes epithelial-mesenchymal transition (EMT), metastasis, and immune evasion . The antibody detects HOXA11 in human, mouse, and rat samples, making it a versatile tool for cross-species studies.
FITC Conjugation: Purpose and Applications
FITC is a green fluorescent dye with excitation/emission wavelengths of ~495/519 nm, ideal for co-localization studies with red-fluorescent markers. The conjugation enhances the antibody’s utility in:
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Immunofluorescence (IF): Visualizing HOXA11 in subcellular compartments (e.g., nucleus, cytoplasm).
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Fluorescence-Activated Cell Sorting (FACS): Quantifying HOXA11-expressing cell populations.
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Immunocytochemistry (ICC): Mapping protein expression in tissue sections.
Cellular Localization Studies
The FITC-conjugated antibody is used to track HOXA11’s dynamic expression in developmental and disease contexts:
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Embryogenesis: Visualizing HOXA11 in the developing uterus and limb buds to study morphogenesis .
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Cancer: Detecting HOXA11 in gastric carcinoma (GC) cells to assess its role in peritoneal metastasis . In co-culture models, HOXA11-overexpressing GC cells induce mesothelial fibrosis, which the antibody helps quantify via IF .
Mechanistic Insights in Cancer
HOXA11 interacts with signaling pathways (e.g., PDGF BB/TGF-β1) to drive fibrosis and immune evasion. For example:
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Immune Escape: HOXA11 upregulates PD-L1 via FOSL1 and PTBP1, enabling tumor evasion of CD8+ T cells. The antibody aids in correlating HOXA11 expression with PD-L1 levels in hypopharyngeal squamous cell carcinoma (HSCC) .
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Metastasis: HOXA11 promotes EMT and anoikis resistance in GC cells, as demonstrated by IF-based studies .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Our research revealed a novel potential regulatory mechanism between HOTTIP and one of its physical HOXA clusters, HOXA11. This suggests that HOTTIP may partially mediate HOXA11 expression, which is involved in cell growth, migration, and apoptosis in breast cancer MCF-7 cells. PMID: 29415429
- Elevated expression of lncRNA HOXA11-AS promotes breast cancer invasion and metastasis by influencing epithelial-mesenchymal transition (EMT). PMID: 28701685
- Silencing HOXA11 suppressed osteosarcoma cell proliferation, invasion, and induced cell cycle arrest. PMID: 28558357
- Our findings indicate that aberrant hypermethylation and the methylation-induced down-regulation of HOXA11 may contribute to lung adenocarcinoma progression. PMID: 28380439
- Hypermethylation of HOXA11 is an independent prognostic biomarker in breast cancer. This suggests HOXA11 as a potential tumor suppressor. PMID: 28038461
- The treatment resistance induced by HOXA11 underexpression can contribute to a poor prognosis in glioblastoma multiforme (GBM). PMID: 27456940
- Our results indicate that mutations in the coding sequence of HOXA11 might not be a common cause of cryptorchidism. However, common polymorphisms in the HOXA11 gene might contribute to the risk of developing unilateral cryptorchidism. PMID: 28261830
- Our research demonstrated that HOXA11-antisense (AS) functioned as a molecular sponge for miR-124, and overexpression of miR-124 attenuated the proliferation and invasion-promoting effect of HOXA11-AS. PMID: 28749709
- Bioinformatics prediction indicated that miR-140-5p directly targeted HOXA11-antisense RNA at the 3'-UTR, which was confirmed by luciferase reporter assay. PMID: 28832185
- HOXA11-AS functions as a competing endogenous RNA. PMID: 29034803
- Our study demonstrated that HOXA11 acts as a tumor suppressor in renal cell carcinoma. PMID: 28423531
- Laparoscopic endometrioma resection increases peri-implantation endometrial HOXA-11 mRNA expression. PMID: 26056923
- Epigenetic silencing of HOXA11 promotes gastric cancer proliferation, migration, and invasion through activation of Wnt signaling. PMID: 25590359
- Overexpression of miR-30d or 181a suppressed HOXA11 mRNA. PMID: 25630974
- Low HOXA11 expression is associated with hepatocellular carcinoma. PMID: 24306662
- Mutations in the coding region of HOXA11 are not common in Chinese women with Mullerian duct anomalies. PMID: 24745830
- Our study suggests that HOXA11 hypermethylation may contribute to the progression of non-small cell lung cancer (NSCLC) by promoting cell proliferation or migration. PMID: 24259349
- Lower expression of HOXA11 is associated with endometriosis-associated infertility. PMID: 23979130
- Promoter methylation of HOXA11 is associated with glioblastoma. PMID: 23624749
- Reduced HOXA11 expression may contribute to endometriosis-associated infertility. PMID: 22233680
- Endometrial HOXA11 and LIF mRNA expression levels (normalized to beta-actin expression) were significantly decreased in the endometrium of infertile patients with endometriosis compared with healthy fertile controls. PMID: 21987111
- Our results suggest that HOXA-11 gene expression in the endometrium during the implantation window may not be altered in patients with idiopathic infertility. PMID: 21526497
- Expression of HOXA11 represses the expression of p53 in uterosacral ligaments. PMID: 19372592
- Our findings suggest that altered HOXA11 gene expression in the endometrium during a menstrual cycle may be a common phenomenon among patients with endometriosis and may cause infertility in this group of patients. PMID: 20695189
- NUP98 primer and a degenerate primer corresponding to the third helix of the homeodomain of HOXA demonstrated that NUP98 was fused in-frame to HOXA11 in the patient with chronic myelogenous leukemia (CML). PMID: 11830496
- HOXA11 is expressed in the developing kidney. PMID: 12060755
- HOXA gene expression is up-regulated at the ectopic implantation site in the fallopian tube and it may play a role in ectopic pregnancies. PMID: 15167850
- Our study reveals a novel expression pattern for homeobox A11 (HOXA11) gene in human endometrium. PMID: 15467538
- The HOXA11 gene cluster is deregulated in T-cell malignancies, resulting in abnormal HOXA gene expression. This finding is consistent with a previous report suggesting HOXA deregulation in MLL-rearranged T cell lymphoblastic leukemia. PMID: 15674412
- HOXA11 is essential for the development and maintenance of uterosacral ligaments and is deficient in pelvic prolapse. PMID: 18274672
- Our findings do not support the hypothesis that mutations in the HOXA11 coding regions are involved in the pathogenesis of human non-syndromal congenital renal parenchymal malformations. PMID: 19255789
- Methylation of the HOXA11 gene is associated with high-grade squamous intraepithelial lesions in cervical cancer. PMID: 19609949
