HIRD11 Antibody

Biological Role of HIRD11

HIRD11 promotes plant growth, particularly influencing primary root elongation. Mutants lacking functional HIRD11 exhibit stunted growth, with primary roots 30–40% shorter than wild-type plants . Complementation experiments restoring HIRD11 expression rescue this phenotype, confirming its critical role in development .

Table 1: Phenotypic Effects of HIRD11 Mutations

Regulation by RNA G-Quadruplex (RG4) Structures

HIRD11’s translation is modulated by an RG4 structure in its 3’ untranslated region (UTR) :

• RG4 Function: This guanine-rich sequence forms a stable secondary structure in vivo, suppressing translation under normal conditions .

• Mutation Impact: Disrupting the RG4 (mutRG4) increases polysome-associated HIRD11 mRNA by 60%, enhancing protein synthesis and unexpectedly elongating roots beyond wildtype levels .

Table 2: RG4-Dependent Translational Regulation

Challenges in Antibody Development

Despite HIRD11’s functional importance, no antibody has been commercialized due to:

• Technical Limitations: High sequence similarity among dehydrins complicates epitope specificity .

• Alternative Methods: Studies rely on polysome profiling and qRT-PCR to quantify translational efficiency .

Future Directions

• Antibody Engineering: Custom monoclonal antibodies could enable precise protein localization and quantification.

• Agricultural Applications: Modulating RG4 structures might enhance stress tolerance in crops by fine-tuning dehydrin expression .