Customize HSP17.7 Antibody

Description

Introduction to HSPAntibody

HSP17.7 is a class II cytosolic small heat shock protein (smHSP) predominantly found in plants, including Arabidopsis thaliana and Daucus carota (carrot). These proteins are critical for cellular stress responses, acting as molecular chaperones to prevent protein denaturation under heat stress . The HSP17.7 antibody is a polyclonal tool developed to detect and study this protein’s expression, localization, and functional mechanisms in plant systems.

Development and Specificity

The HSP17.7 antibody is generated using full-length recombinant HSP17.7 protein produced in Escherichia coli and purified without affinity tags . Key characteristics include:

Host species: Rabbit
Clonality: Polyclonal
Reactivity: Primarily plants (Arabidopsis thaliana, Cucumis sativus, Solanum vulgaris)
Specificity: Recognizes a ~17.7 kDa monomer and an SDS-resistant dimer (~38 kDa) .

The antibody does not cross-react with class I smHSPs, enabling precise differentiation between smHSP subfamilies .

Western Blotting

Detects endogenous HSP17.7 in heat-stressed Arabidopsis tissues at dilutions of 1:1,000 .
Validates recombinant HSP17.7 expression in transgenic E. coli, demonstrating its role in thermotolerance .

Functional Studies

Used to investigate HSP17.7’s chaperone activity in stabilizing lipids and denatured proteins .
Confirms distinct complex formation between class I and II smHSPs via non-denaturing electrophoresis .

Table 1: HSP17.7 Antibody in Experimental Models

Study Focus	Key Outcome	Citation
Thermotolerance in E. coli	HSP17.7-expressing cells showed 4x higher viability at 50°C vs. controls
smHSP Complex Differentiation	Class II smHSPs form distinct 8S complexes, separate from class I smHSPs
Dimer Detection	Antibody identifies SDS-resistant HSP17.7 dimers in heat-stressed plants

Mechanistic Insights

HSP17.7 stabilizes lipid membranes and collaborates with the DnaK/DnaJ/GrpE and GroEL/ES chaperone systems for protein refolding .
In Arabidopsis, HSP17.7 induction correlates with heat shock transcription factor activation, confirming stress-responsive regulation .

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

HSP17.7 antibody; At5g12030 antibody; F14F18.20017.7 kDa class II heat shock protein antibody; 17.7 kDa heat shock protein antibody; AtHsp17.7 antibody

Target Names

HSP17.7

Uniprot No.

O81822

Target Background

Gene References Into Functions

A novel peroxisome-located catalase chaperone involved in plant abiotic stress resistance by activating catalase activity. PMID: 28869112

Database Links

KEGG: ath:AT5G12030

STRING: 3702.AT5G12030.1

UniGene: At.21308

Protein Families

Small heat shock protein (HSP20) family

Subcellular Location

Cytoplasm.

Q&A

Here’s a structured FAQ collection for researchers working with HSP17.7 antibodies, synthesized from peer-reviewed literature and technical reports:

Advanced Research Questions

Resolving contradictions in HSP17.7’s dual role as a chaperone and aggregase
- Analysis Framework:
  
  Substrate HSP17.7 Activity Conditions Citation
  MDH Aggregase 41°C, 1:2 ratio
  GAPDH Anti-aggregase 16–32x excess
  - Solution: Test multiple substrates under varying stoichiometries and temperatures to contextualize functional duality .
Interpreting age-dependent solubility shifts of HSP17.7 in longitudinal studies
- Strategy:
  - Fractionate soluble/insoluble proteins via centrifugation at different life stages (e.g., day 2 vs. day 8 C. elegans).
  - Correlate HSP17.7 insolubility with aging markers (e.g., proteostatic decline) .
Can anti-HSP17.7 titers serve as biomarkers for heat stress adaptation?
- Data-Driven Workflow:
  - Quantify antibody titers via immunoblotting in heat-stressed models (e.g., human patients or C. elegans).
  - Monitor titer changes during recovery phases; higher titers correlate with severe stress exposure .

Substrate	HSP17.7 Activity	Conditions	Citation
MDH	Aggregase	41°C, 1:2 ratio
GAPDH	Anti-aggregase	16–32x excess

Methodological Best Practices

For Aggregation Assays:
Use light-scattering kinetics (e.g., 500 nm MDH + 2x HSP17.7) to quantify aggregase activity. Include human HSPB5 as a canonical anti-aggregase control .
Longitudinal Studies:
Synchronize model organisms (e.g., C. elegans) to control for developmental variability in HSP17.7 expression .

Key Data Contradictions & Solutions

Contradiction: HSP17.7 depletion reduces lifespan but delays intestinal protein aggregation .
- Resolution: Distinguish between systemic stress tolerance (lifespan) and compartment-specific aggregation kinetics.
Contradiction: Anti-HSP antibodies appear in healthy individuals but spike during heat stress .
- Resolution: Use age-/environment-matched controls and normalize titers to baseline levels.

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HSP17.7 Antibody