HST Antibody

HST Antibody Targets and Biological Contexts

HST (FGF4)

• Gene: FGF4 (Human)

• Protein: 206-amino acid secretory protein regulating embryonic development, cell proliferation, and differentiation .

• Antibody Types:

  • Monoclonal antibodies (e.g., HS-131, HS-276) targeting epitopes at amino acid residues 59–73 and 197–206 .

  • Polyclonal antibodies for Western blot (WB) and ELISA .

HST (Homogentisate Prenyltransferase)

• Gene: HST (Arabidopsis thaliana)

• Function: Catalyzes phytyl transfer in vitamin E biosynthesis .

• Antibodies: Rabbit polyclonal antibodies validated for WB and ELISA .

HST (Hydroxysteroid Sulfotransferase, SULT2A1)

• Gene: SULT2A1 (Human)

• Function: Sulfates steroids like dehydroepiandrosterone (DHEA) .

• Antibodies: Rabbit IgG antibodies for WB and ELISA .

Cancer and Immunotherapy

• FGF4 (HST-1): Adenovirus-mediated HST-1 gene transfer increased platelet counts in murine models, suggesting thrombopoietic potential .

• Monoclonal Antibodies: HS-233 and HS-131 antibodies neutralized HST-1 activity and enabled sensitive detection (1.2–2.5 pg/well) via sandwich ELISA .

HIV Research

• Recombinant HST Carrier: Engineered HST proteins delivered HIV protease substrates but elicited weak immune responses compared to peptide-HST conjugates .

• Antibody-Dependent Cellular Cytotoxicity (ADCC): T cell-secreted anti-HIV antibodies (e.g., 10-1074) bound Env-expressing cells and enhanced NK cell killing by 28% .

Plant Biology

• Vitamin E Biosynthesis: Anti-HST antibodies identified homogentisate prenyltransferase in Arabidopsis, linking HST to chloroplast function and stress responses .

Validation Challenges

• Specificity Issues: Cross-reactivity remains a concern, as seen in the Human Protein Atlas’s reliance on antibody providers’ quality control .

• Best Practices: Use antigen-affinity purification and validate via multiple methods (e.g., WB, ELISA, neutralization assays) .