IAA16 Antibody

Biological Role of IAA16 in Plant Signaling

IAA16 is a member of the Aux/IAA protein family, which regulates auxin-mediated gene expression by interacting with AUXIN RESPONSE FACTORs (ARFs). Key findings include:

Mutational Analysis

• A gain-of-function mutation (iaa16-1) alters the GWPPV degron motif in domain II, stabilizing the protein and conferring:

  • Auxin resistance: Reduced root elongation inhibition by IAA and 2,4-D .

  • ABA resistance: Impaired abscisic acid responsiveness during seedling establishment .

  • Developmental defects: Severe stunting, infertility in homozygous plants, and reduced apical dominance .

Interaction Networks

• Phosphorylation: Unlike IAA8, IAA16 is not phosphorylated by stress-activated MPK3/6 kinases, suggesting distinct regulatory mechanisms .

• Protein-Protein Interactions: IAA16 interacts with ARF10/18 in transcriptional repression, as shown via bimolecular fluorescence complementation assays .

Comparative Analysis of Aux/IAA Proteins

Research Applications of IAA16 Antibody

• Co-Immunoprecipitation (Co-IP): Used to validate interactions between IAA16-Myc and TIR1-Myc in Arabidopsis protoplasts .

• Expression Analysis: Quantifies IAA16 levels in transgenic lines overexpressing ArAux/IAA16 (e.g., 3.5-fold higher in OE lines vs. wild type) .

• Subcellular Localization: Confirms nuclear localization of IAA16-GFP fusion proteins .

Key Studies Utilizing IAA16 Antibody

1. Stress Signaling: Demonstrated that MPK3/6-mediated phosphorylation does not target IAA16, unlike IAA8, highlighting functional divergence in auxin signaling under stress .

2. Transgenic Phenotyping: Revealed that overexpression of ArAux/IAA16 reduces adventitious root formation by 40–60% in Arabidopsis .

3. Hormone Crosstalk: Linked auxin-ABA crosstalk through iaa16-1 mutants, showing dual resistance to both hormones .