IAA17 Antibody
Description
Overview of IAA17 Antibody
The IAA17 antibody is a specialized immunological tool designed to detect and study the Auxin-Responsive Protein IAA17 (Indole-3-Acetic Acid 17), a key player in auxin signaling pathways in plants. This antibody is primarily used in research to investigate IAA17's role in plant growth, stress responses, and protein-protein interactions .
Target Protein: IAA17
IAA17 belongs to the Aux/IAA family of nuclear proteins, which regulate auxin-mediated gene expression by interacting with Auxin Response Factors (ARFs). These short-lived proteins are stabilized by mutations in conserved domains (e.g., axr3-1 mutation in domain II), leading to altered auxin responses .
Recombinant Protein Generation
IAA17 antibodies are typically generated using recombinant IAA17 proteins produced in eukaryotic systems. For example:
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Host system: Yeast (Saccharomyces cerevisiae) for cost-effective expression with post-translational modifications .
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Tag: 6× His-tagged IAA17 (amino acids 1–229 or 1–257) for purification and antibody specificity .
Protein Stability and Degradation Studies
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Half-life assays: The iaa17/axr3-1 mutant protein shows a 7× longer half-life (~550 min) compared to wild-type IAA17 (~80 min) .
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NO-mediated stabilization: Nitric oxide (NO) donors (e.g., GSNO) inhibit IAA17 degradation, increasing its accumulation in mutants like nox1 and gsnor1-3 .
Subcellular Localization
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Nuclear targeting: IAA17 contains conserved nuclear localization signals (NLS). Transient expression assays confirm nuclear accumulation of wild-type and mutant IAA17 (e.g., axr3-1R2) .
Protein-Protein Interactions
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Heterodimerization: IAA17 interacts with ARF1/ARF5 and other Aux/IAA proteins (e.g., IAA3/SHY2). Revertant mutations in domains I/III disrupt these interactions .
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SCF<sup>TIR1</sup> complex: IAA17 degradation is mediated by the E3 ubiquitin ligase TIR1. S-nitrosylation at Cys-15/Cys-70 reduces TIR1 binding, stabilizing IAA17 .
Table 2: Key Research Applications
Mutation-Induced Phenotypes
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Domain II mutations: The iaa17/axr3-1 gain-of-function mutation (Pro81Ser) stabilizes the protein, causing auxin hypersensitivity .
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Intragenic suppressors: Revertants like axr3-1R2 (Asp119Asn) restore wild-type phenotypes by reducing homodimerization capacity .
Stress Response Mechanisms
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Salt stress: NO stabilizes IAA17 via S-nitrosylation, enhancing salt tolerance in Arabidopsis .
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Crosstalk with gibberellins: IAA17 interacts with RGL3 to repress GA biosynthesis genes (e.g., GA3ox), linking auxin and stress signaling .
Technical Considerations for IAA17 Antibody Use
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Antibody validation: Specificity confirmed via immunoblotting against recombinant IAA17 and knockout mutants .
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Buffer compatibility: Tris-based buffer with 50% glycerol for long-term stability .
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Limitations: Low endogenous IAA17 abundance necessitates sensitive detection methods (e.g., in vivo labeling) .
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research indicates that AtIAA17 is a positive regulator of natural leaf senescence and establishes a direct link between melatonin and AtIAA17 in the process of natural leaf senescence in Arabidopsis. PMID: 25324183
- icu6 is a novel mutant allele of AXR3 that results in severe leaf incurvature, as well as heightened responsiveness to auxin and growth defects in the adaxial domain of leaves. PMID: 20739302
- AXR3/IAA17 might be involved in the brassinosteroid (BR) signaling pathway, suggesting a point of intersection between BR-auxin signaling in root development. PMID: 16636440
- PAX1 influences auxin response through its effects on AXR3 expression. PMID: 17430601
