IAA27 Antibody

Biological Function of IAA27

IAA27 plays a critical role in modulating plant responses to environmental stressors, particularly phosphorus deficiency. Key findings include:

• Adventitious root development: Overexpression of MdIAA27T (a specific allele in apple) enhanced root length and density under low-phosphorus conditions, improving phosphorus uptake .

• Allelic variation: Two linked SNPs in MdIAA27 (C vs. T alleles) caused divergent phenotypes:

  • MdIAA27T promoted root growth and phosphorus absorption.

  • MdIAA27C suppressed root development .

• Subcellular localization: IAA27 localizes to the nucleus, consistent with its role in transcriptional regulation .

Molecular Interactions

IAA27 interacts with multiple partners to mediate auxin signaling:

Table 1: Key Interaction Partners and Regulatory Targets

• ARF binding: Under low phosphorus, IAA27 degradation releases ARF26/27 to activate genes like SAUR76 and LBD16, driving root growth .

• Pathogen interaction: The Tobacco Mosaic Virus (TMV) replicase disrupts IAA27 nuclear localization, potentially interfering with auxin signaling during infection .

Research Applications of IAA27 Antibody

The IAA27 antibody is critical for:

1. Protein localization studies: Detecting nuclear vs. cytoplasmic shifts in IAA27 during stress or pathogen infection .

2. Functional allelic analysis: Distinguishing between MdIAA27T and MdIAA27C isoforms in transgenic plants .

3. Interaction assays: Validating IAA27-ARF or IAA27-pathogen protein complexes via immunoprecipitation .

Table 2: Phenotypic Effects of IAA27 Overexpression