IAA33 Antibody

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Description

Functional Mechanisms

IAA33 modulates auxin responses through competitive binding with ARF transcription factors:

MechanismExperimental EvidenceCitation
ARF10/16 interactionCo-IP and BiFC confirm direct binding to ARF10/16, critical for root stem cell maintenance
Competition with IAA5In vitro pull-down assays show IAA33 displaces IAA5 from ARF10/16 complexes
Auxin response regulationOverexpression reduces DR5rev::GFP signal by 60% compared to wild type

Role in Plant Development

IAA33 maintains root distal stem cell (DSC) identity through ARF10/16:

  • Mutant phenotypes:

    • iaa33 mutants show 42% increase in 2-layer DSC structures

    • Overexpression lines exhibit premature DSC differentiation

  • Genetic interactions: DSC defects rescued by ARF10/16 mutations

Antibody Applications in IAA33 Research

While no commercial IAA33-specific antibody is documented, studies utilize epitope-tagged versions:

ApplicationTag SystemKey Findings
Co-immunoprecipitationMYC/GFP tagsConfirmed IAA33-ARF10/16 interactions in tobacco leaves
Subcellular localizationGUS-GFP fusionDetected IAA33 expression in root tips via fluorescence microscopy
Protein stability assaysMYC immunoblottingShowed auxin stabilizes IAA33 (2.3-fold increase after 2h NAA treatment)

Comparative Analysis with Canonical AUX/IAA Proteins

FeatureIAA33 (Non-canonical)Canonical AUX/IAA (e.g., IAA5)
TIR1 bindingNo interactionStrong TIR1 affinity
Auxin-induced degradationStabilized by auxinRapidly degraded
ARF binding partnersPreferentially binds repressor ARFs (ARF1,10,16,18)Binds both repressor/activator ARFs

Product Specs

Buffer
**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
IAA33 antibody; At5g57420 antibody; MUA2.1 antibody; Auxin-responsive protein IAA33 antibody; Indoleacetic acid-induced protein 33 antibody
Target Names
IAA33
Uniprot No.

Target Background

Function
Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to occur through interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may modulate their ability to regulate early auxin response gene expression.
Database Links

KEGG: ath:AT5G57420

STRING: 3702.AT5G57420.1

UniGene: At.55603

Protein Families
Aux/IAA family
Subcellular Location
Nucleus.

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