IAN1 Antibody
Description
IAN1: Overview and Function
The Ian gene family encodes small GTPases implicated in immune regulation. Ian1 (also called Immune-Associated Nucleotide-Binding Protein 1) is expressed in lymphoid tissues and macrophages, with roles in T-cell development and immune responses .
IAN1 Antibody: Research Applications
A polyclonal antiserum against Ian1 was developed for Western blotting, revealing reduced Ian1 protein levels in T cells from autoimmune-prone lyp/lyp rats compared to wild-type .
Table 1: Ian Gene Expression in Rat Cells (Relative Quantification)
| Cell Type | Ian1 | Ian5 | Ian6 |
|---|---|---|---|
| LN T Cells (Wild-Type) | 100 | 507 | 54 |
| Peritoneal Macrophages | 49 | 310 | 2197 |
| Thymocytes | 50 | 4438 | 104 |
Key Findings:
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Ian1 is highly expressed in wild-type LN T cells but diminished in lyp/lyp mutants .
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Ian1 levels correlate with T-cell survival and autoimmune disease susceptibility .
Potential Confusion: Iba1 vs. Ian1
Most commercial antibodies labeled "IBA1" target Ionized Calcium-Binding Adapter Molecule 1 (AIF1), a macrophage/microglia marker unrelated to Ian1. Examples include:
Research Implications
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IAN1 Antibodies are niche tools for studying T-cell biology and autoimmune mechanisms but lack extensive commercial availability.
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Iba1 Antibodies dominate research due to their utility in neuroinflammation and macrophage studies .
Limitations and Future Directions
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No commercial monoclonal antibodies specific to Ian1 are currently documented.
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Further studies are needed to elucidate Ian1’s structural epitopes and therapeutic potential in autoimmune diseases.
Product Specs
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
