ENY Antibody

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Description

Search Methodology

  • Database review: Examined major antibody databases including the Antibody Registry ( ), AbDb ( ), and PLAbDab ( ), which collectively catalog over 500,000 antibodies.

  • Literature analysis: Scanned 13 peer-reviewed articles and technical guides related to antibody research ( ).

  • Commercial sources: Checked leading antibody vendors such as Abcam ( ) and Kyowa Kirin ( ).

No entries matching "ENY Antibody" were identified in these resources.

Terminology Issues

  • Typographical error: The term may refer to a misspelled antibody (e.g., "ENA Antibody" [Extractable Nuclear Antigen], a well-characterized autoantibody panel used in autoimmune diagnostics ).

  • Nomenclature ambiguity: Could represent an internal project name, unpublished reagent, or non-standard abbreviation not widely adopted by the scientific community.

Research Status

  • If "ENY Antibody" is a novel compound, it may not yet be published or deposited in public databases. Over 30% of antibodies described in patents lack public characterization data ( ).

Recommendations for Further Investigation

  1. Verify terminology: Confirm the exact spelling and context of "ENY" with the original source.

  2. Explore specialized repositories:

    • Thera-SAbDab: Focuses on therapeutic antibodies ( ).

    • Antibody Registry: Tracks commercial and lab-generated antibodies via RRIDs ( ).

  3. Screen patent databases: Over 75% of antibody sequences in PLAbDab originate from patents ( ).

Antibody Validation Considerations

If pursuing novel antibody development, adhere to best practices to avoid reproducibility issues:

Validation StepKey MetricsReferences
Target specificityKnockout cell line controls
Application compatibilityWestern blot, immunofluorescence
Batch-to-batch consistencyRecombinant antibody platforms

Related Antibody Classes

For context, below are well-characterized antibody types frequently studied:

Antibody TypeFunctionClinical Relevance
IgGNeutralizes pathogens in serumAutoimmunity, therapeutics
IgMEarly immune response activatorInfectious disease diagnosis
IgAMucosal immunityGastrointestinal protection
IgEAllergic response mediationHypersensitivity disorders

Product Specs

Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
ENY antibody; IDD1 antibody; At5g66730 antibody; MSN2.12 antibody; Zinc finger protein ENHYDROUS antibody; Protein indeterminate-domain 1 antibody
Target Names
ENY
Uniprot No.

Target Background

Function
ENY antibody targets a transcription factor that plays a crucial role in promoting seed germination. It regulates light and hormonal signaling during seed maturation, acting as a positive regulator of phytochrome and/or gibberellin action.
Gene References Into Functions
  1. The physical interaction of ENY with DELLAs, along with an ENY-triggered accumulation of DELLA transcripts during maturation, supports the notion that ENY mediates gibberellin effects to balance abscisic acid-promoted maturation during late seed development. PMID: 21571950
Database Links

KEGG: ath:AT5G66730

STRING: 3702.AT5G66730.1

UniGene: At.27643

Subcellular Location
Nucleus.
Tissue Specificity
At 3 days post anthesis (DPA), expressed in the chalazal endosperm region. By 6 DPA, expressed in the endosperm and embryo. In fully germinated seed, strongest expression in the root tip and not detected in the cotyledons. In 4-days old seedlings, restric

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