At3g57470 Antibody

Overview of At3g57470 Antibody

The At3g57470 Antibody (Product Code: CSB-PA943883XA01DOA) targets the protein product of the At3g57470 gene in Arabidopsis thaliana (Mouse-ear cress). Key specifications include:

This antibody is part of a catalog offering tools for plant molecular biology research, focusing on proteins involved in stress responses, metabolism, and development .

Biological Context of the At3g57470 Protein

The At3g57470 gene encodes a protein of unknown function, as annotated in UniProt. Homology analyses suggest it may belong to a conserved plant-specific protein family. Antibodies like CSB-PA943883XA01DOA enable researchers to:

• Localize the protein within tissues (e.g., via immunofluorescence).

• Quantify expression under different experimental conditions (e.g., drought, pathogen exposure) .

Research Applications and Challenges

While no direct studies using this antibody are cited in the provided sources, insights can be extrapolated from analogous plant antibody research:

Key Technical Considerations

• Specificity: Antibodies against plant proteins require rigorous validation due to high sequence homology among plant gene families .

• Cross-reactivity: Epitope mapping is critical; the Y-shaped structure of antibodies allows precise binding, but plant secondary metabolites may interfere with assays .

Comparative Data

A 2023 study on antibody engineering highlighted methods to enhance binding avidity through noncovalent catenation, a technique potentially applicable to low-abundance plant targets like At3g57470 . Additionally, advancements in antibody-dependent cellular cytotoxicity (ADCC) assays underscore the importance of robust validation protocols for plant research reagents.

Future Directions

• Functional Studies: Linking At3g57470 to pathways using knockout mutants and antibody-based localization.

• Technical Optimization: Adopting phage display or computational design to improve antibody affinity for challenging plant targets.