dyf-6 Antibody
Description
Introduction to DYF-6
DYF-6 is a protein encoded by the dyf-6 gene in Caenorhabditis elegans (C. elegans). It is a component of the intraflagellar transport (IFT) Complex A, which is critical for the assembly and maintenance of cilia—a cellular structure essential for sensory and signaling functions . DYF-6 homologs are conserved across species; in humans, its counterpart is IFT46, a protein involved in ciliogenesis and associated with diseases like Bardet-Biedl syndrome .
Functional Role of DYF-6
DYF-6 (IFT46) facilitates retrograde transport within cilia by regulating the movement of IFT particles along microtubules. Key findings include:
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Genetic Interactions: DYF-6 interacts with other IFT components such as IFT144 (FLA15) and IFT139 (FLA17) to ensure proper ciliary assembly .
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Mutant Phenotypes: dyf-6 mutants exhibit defects in cilia structure, leading to sensory neuron dysfunction (e.g., impaired chemotaxis and mechanosensation) .
DYF-6 Antibody: Applications and Research
While no commercially available "DYF-6 antibody" is explicitly detailed in the provided sources, studies on IFT46 (the mammalian homolog) and related C. elegans proteins suggest potential applications:
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Localization Studies: Antibodies against DYF-6 could track its distribution in ciliated neurons or glial cells.
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Functional Assays: Used to investigate DYF-6’s role in genetic pathways, such as its interaction with DAF-6 (a patched-related protein) and DYF-4 (a secreted glial factor) .
Table 1: DYF-6 Genetic and Protein Interactions
| Protein | Function | Interaction Partners | Phenotype in Mutants |
|---|---|---|---|
| DYF-6 (IFT46) | Retrograde IFT in cilia | IFT144, IFT139, DAF-6 | Ciliary defects, sensory loss |
| DAF-6 | Glial channel formation | DYF-4, WSP-1, LIT-1 | Dendrite truncation |
| DYF-4 | Secreted glial regulator | DAF-6 | Defective ciliogenesis |
Table 2: IFT Complex Composition in C. elegans
| Complex | Component | C. elegans Gene | Function |
|---|---|---|---|
| A | IFT46 | dyf-6 | Retrograde transport regulation |
| A | IFT144 | fla15 | Particle remodeling |
| B | IFT172 | osm-1 | Anterograde transport |
Molecular Mechanisms
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DYF-6 and DAF-6 Pathway: DYF-4, a secreted protein, regulates DAF-6 localization in glial cells, indirectly influencing DYF-6-mediated ciliary functions .
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Suppressor Effects: Mutations in wsp-1, lit-1, or igdb-2 restore ciliary defects in dyf-6 mutants, indicating shared pathways with DAF-6 .
Implications for Disease
Defects in DYF-6/IFT46 are linked to ciliopathies, including:
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Retinal degeneration (via disrupted photoreceptor cilia).
Future Directions
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Antibody Development: Custom antibodies against DYF-6 could enable precise studies of its structural dynamics in vivo.
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Therapeutic Targets: Modulating DYF-6 interactions may address cilia-related disorders.
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
Customer Reviews
Applications : Immunoblotting analysis
Sample type: human
Review: The protein level of TTBK2, BBS4, SEPTIN2, and IFT46 was dramatically decreased along with the gradient increased ORF10 expression. Immunoblotting analysis of TTBK2, BBS4, SEPTIN2, IFT46, and ORF10 levels in ORF10-expressing and empty vector (NC) control HEK293T cells. GAPDH served as a loading control (J). Odyssey-based quantification of protein levels in J (n = 3 independent experiments). Data are presented as the mean ± SEM. Statistical analysis was performed with two-tailed unpaired Student’s t test (K). Cells transfected with pcDNA-ORF10-HA display reduced level of multiple cilium-related proteins. Immunoblotting analysis of TTBK2, BBS4, SEPTIN2, IFT46, and ORF10 levels in ORF10-expressing and empty vector (NC) control HEK293T cells. GAPDH served as a loading control (L) .
