IL 1 Beta Antibody, Biotin
Description
Key Applications in Research
These antibodies are primarily used in sandwich ELISA, ELISPOT, and flow cytometry to study IL-1β's role in inflammation, autoimmune diseases, and host defense .
Common Pairings for ELISA
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Capture Antibodies: Purified anti-IL-1β (e.g., CRM56 for human IL-1β)
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Detection Antibodies: Biotinylated clones (e.g., JK1B-2, CRM57)
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Standards: Recombinant IL-1β (e.g., 8–1,000 pg/mL dynamic range)
In Vivo Studies
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Disease Models: Neutralizing IL-1β in mouse models of rheumatoid arthritis and CAPS (cryopyrin-associated periodic syndromes) reduced inflammation .
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Therapeutic Monitoring: Canakinumab (anti-IL-1β) lowered IL-1β production rates from 31 ng/day to normal levels in CAPS patients .
Mechanistic Insights
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IL-1β secretion depends on caspase-1-mediated cleavage and non-classical secretion pathways .
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Autocrine amplification of IL-1β production was observed in CAPS, creating a feedback loop .
Therapeutic Antibody Development
*Novel antibody showed 10× higher potency than canakinumab in neutralizing IL-1β .
Optimization Parameters
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Biotin:Antibody Ratio: Critical for signal-to-noise ratios; excess biotin causes nonspecific binding .
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Blocking Buffers: Required to minimize background in streptavidin-HRP systems .
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Storage: Stable at 4°C for 6 months; avoid freeze-thaw cycles .
Limitations
Product Specs
Product Science Overview
Mouse Anti-Human Biotin antibodies are monoclonal antibodies developed in mice that are specific to human antigens and conjugated with biotin . Biotin, also known as Vitamin B7, is a ubiquitous co-factor with many properties that make it extremely useful for molecular biology applications .
Applications and Uses
Mouse Anti-Human Biotin antibodies are widely used in various scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA, and Flow Cytometry . These antibodies can detect human IgG1 and other immunoglobulin isotypes, making them valuable tools for research and diagnostic purposes .
The antibodies are typically purified from tissue culture supernatant or ascites by affinity chromatography and conjugated with biotin under optimal conditions . They are used in sandwich ELISA assays, where biotin-conjugated antibodies serve as detecting antibodies in combination with capture antibodies .
