IL19 Human, HEK
Description
Biological Function and Signaling Pathways
IL-19 signals through two receptor complexes :
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IL-20 Type I Receptor: IL-20Rα/IL-20Rβ for IL-19, IL-20, and IL-24
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IL-20 Type II Receptor: IL-22Rα1/IL-20Rβ for IL-20 and IL-24
Key Mechanisms:
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STAT3 Activation: IL-19 binding induces JAK/STAT3 phosphorylation, driving anti-inflammatory gene expression (e.g., IL-10) .
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HO-1 Induction: Upregulates heme oxygenase-1 (HO-1) in vascular smooth muscle cells (VSMCs), reducing ROS and apoptosis .
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Immune Polarization: Promotes Th2 over Th1 differentiation, suppressing pro-inflammatory cytokines like TNF-α and IL-6 .
Vascular Protection
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HO-1 Mediated Effects: IL-19 increases HO-1 mRNA/protein in VSMCs by 5-fold within 4 hours, reducing oxidative stress (35.1% ROS reduction) .
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Atherosclerosis: Elevated IL-19 correlates with arterial protection, inhibiting plaque formation via STAT3-dependent pathways .
Inflammatory Regulation
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Monocyte Modulation: Enhances IL-10 production in dendritic cells and monocytes .
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Therapeutic Potential: Reduces severity in acute myocardial infarction (STEMI) and psoriasis models .
Detection and Bioassay Systems
HEK-Blue™ reporter cells enable IL-19 quantification through SEAP secretion :
| Parameter | HEK-Blue IL-19/IL-20 Cells |
|---|---|
| Detection Range | 3–100 ng/mL |
| Specificity | IL-19, IL-20, IL-24 |
| Signal Readout | STAT3-driven SEAP activity |
This system excludes cross-reactivity with IL-6, IL-10, or IFN-α/β .
Clinical Relevance
Product Specs
Melanoma differentiation association like protein, MDA1, NG.1, ZMDA1, IL-10C, IL-19
HEK293 cells.
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Product Science Overview
IL-19 is preferentially expressed in monocytes, macrophages, and T and B lymphocytes . It is produced by B cells and monocytes and binds to the interleukin 20 receptor complex to activate STAT3 signaling . The production of IL-19 is up-regulated in resting monocytes following stimulation with granulocyte-macrophage colony-stimulating factor (GM-CSF) or lipopolysaccharide (LPS) .
IL-19 plays a significant role in the immune response by promoting T helper 2 (Th2) cell-mediated immune responses . It induces the expression of IL-6 and tumor necrosis factor alpha (TNF-α) in monocytes . IL-19 initiates the JAK-STAT signaling pathway, which activates genes and creates mRNA sequences that are translated into proteins with downstream effector functions . The signaling involves the IL-20 dimer receptor complexes that bind the IL-19 ligand, Janus kinases (JAKs), and the signal transducer and activator of transcription 3 (STAT3) .
Recombinant IL-19 (Human Recombinant, HEK) is produced in HEK 293 cells. It is typically used in research settings to study its effects on immune responses and its potential therapeutic applications . The recombinant form of IL-19 is available in various purities and formulations, and it is often lyophilized from a sterile filtered solution containing trifluoroacetic acid (TFA) .
IL-19 has broad functions across inflammation, cell development, viral responses, and lipid metabolism . As an immunosuppressive cytokine, IL-19 promotes an anti-inflammatory lymphocyte phenotype, dampens the Th1 T-cell response, and increases IL-10 expression in peripheral blood mononuclear cells (PBMC) . It also inhibits the production of immunoglobulin G (IgG) from B cells .
