IL1A Mouse, Sf9
Description
Introduction to IL-1α and Sf9 Expression System
IL-1α (Interleukin-1 alpha) is a proinflammatory cytokine critical for innate immune responses, inflammation, and cell death signaling. Produced by macrophages, epithelial cells, and endothelial cells, it activates immune cells and regulates fever, sepsis, and tissue damage responses . The Sf9 baculovirus system is a robust platform for producing recombinant proteins, particularly for glycosylated proteins requiring post-translational modifications. IL-1α Mouse, Sf9 refers to recombinant mouse IL-1α expressed in Sf9 insect cells, enabling proper folding and functional activity for research applications .
Production and Purification of IL1A Mouse, Sf9
Expression System:
Purification Process:
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Cell Lysis: Harvest and lyse infected Sf9 cells.
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Chromatography: Proprietary techniques (e.g., nickel affinity, ion exchange) .
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Quality Control: SDS-PAGE (18–28 kDa band) and RP-HPLC (>90% purity) .
Formulation:
Biological Activity and Relevance in Research
| Assay | Result |
|---|---|
| D10.G4.1 T-Cell Proliferation | ED₅₀ < 0.15 ng/ml |
| KC/CXCL1 Induction | IL-1α-specific chemoattractant expression in macrophages |
| Inflammasome Activation | Independent of IL-1β in Il1a-KO line2 mice |
Applications:
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Inflammatory Disease Models: Studies on autoinflammatory diseases (e.g., chronic IL-1-driven conditions) .
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Therapeutic Targeting: Validating IL-1α-specific inhibitors in sepsis and COVID-19 .
Research Findings and Applications
Key Studies:
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IL-1α vs. IL-1β Specificity:
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Immune Regulation:
Comparison with Other Expression Systems
Advantages of Sf9:
Stability and Handling Recommendations
| Condition | Recommendation |
|---|---|
| Short-Term Storage | 4°C (2–4 weeks) |
| Long-Term Storage | -20°C with 0.1% BSA/HSA carrier protein |
| Freeze-Thaw Cycles | Avoid; aliquot prior to storage |
Reconstitution:
Product Specs
Interleukin 1 alpha (IL-1 alpha), also known as hematopoietin 1, is a cytokine belonging to the interleukin 1 family. Encoded by the IL1A gene in humans, IL-1 alpha plays a crucial role in inflammation, fever, and sepsis. Inhibitors of IL-1 alpha are being developed to modulate these processes for therapeutic purposes. Primarily produced by neutrophils and macrophages, as well as endothelial and epithelial cells, IL-1 alpha binds to the IL-1 receptor, playing a vital role in regulating immune responses. It is also involved in activating tumor necrosis factor-alpha (TNF-alpha) and exhibits physiological, metabolic, and hematopoietic activities.
Recombinant Mouse IL1A, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 165 amino acids (115-270a.a.). With a molecular weight of 19.0 kDa (appears as approximately 18-28 kDa on SDS-PAGE), this IL1A is expressed with a 9 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
The IL1A protein solution, provided at a concentration of 0.5 mg/ml, is prepared in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
For short-term storage (up to 2-4 weeks), store the entire vial at 4°C. For extended storage, freeze the product at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein integrity.
The purity of this product is greater than 90%, as determined by SDS-PAGE analysis.
The ED50, determined using a cell proliferation assay with D10.G4.1 mouse helper T cells, is less than 0.15 ng/ml.
Il1a, Il-1a, Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1.
ADPSAPYTYQ SDLRYKLMKL VRQKFVMNDS LNQTIYQDVD KHYLSTTWLN DLQQEVKFDM YAYSSGGDDS KYPVTLKISD SQLFVSAQGE DQPVLLKELP ETPKLITGSE TDLIFFWKSI NSKNYFTSAA YPELFIATKE QSRVHLARGL PSMTDFQISH HHHHH.
Product Science Overview
IL-1α exerts its effects by binding to specific cell surface receptors, namely IL-1RI and IL-1RII, which are shared with IL-1β. This cytokine is involved in the activation of various signaling pathways that lead to the production of other proinflammatory cytokines and the initiation of acute-phase responses .
IL-1α is a pleiotropic cytokine, meaning it has multiple effects on different cell types. It plays a significant role in:
- Innate Host Defense: Triggering the production of other proinflammatory cytokines in target cells.
- Inflammatory Responses: Initiating and sustaining inflammation.
- Apoptosis: Inducing programmed cell death in response to cell injury .
Recombinant IL-1α is widely used in research to study its role in immune responses and inflammation. It is also utilized in various bioassays to understand its biological activity and interactions with other cytokines and receptors .
The recombinant IL-1α protein is typically lyophilized (freeze-dried) for stability and should be stored desiccated below -18°C. Upon reconstitution, it should be stored at 4°C for short-term use and below -18°C for long-term storage. It is recommended to add a carrier protein, such as 0.1% HSA or BSA, to prevent freeze-thaw cycles .
