IL-1 Alpha Rat, His Active
Description
Biological Activity
IL-1 Alpha Rat, His Active binds to the IL-1 receptor (IL-1R1/IL-1RAP) and activates downstream signaling pathways, including NF-κB and MAPK (p38, JNK, ERK) . Key functional attributes include:
In Vitro Activity
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Immune Cell Activation: Stimulates thymocyte proliferation via IL-2 induction (ED₅₀: <0.005 ng/mL) and B-cell maturation .
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Cytokine Induction: Triggers IL-6, G-CSF, and prostaglandin PGE₂ release in fibroblasts and endothelial cells .
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Bioassay Performance:
In Vivo Activity
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Inflammation: Mediates fever, acute-phase protein synthesis, and neutrophil mobilization .
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Wound Healing: Promotes keratinocyte and fibroblast proliferation via FGF/EGF upregulation .
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Radiation Protection: Enhances survival in γ-irradiated mice through hematopoietic activity .
Key Use Cases
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Immune Response Studies: Investigates IL-1α’s role in LPS-induced fever and sepsis .
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Signal Transduction: Maps NF-κB and MAPK pathways in inflammatory models .
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Therapeutic Development: Tests IL-1 inhibitors for autoimmune diseases .
Experimental Tools
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Neutralization Assays: Validated with anti-IL-1α antibodies (ND₅₀: 0.003–0.009 µg/mL) .
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ELISA Quantification: Detects IL-1α in biological fluids (sensitivity: 16–1,000 pg/mL) .
IL-1α in Peripheral Inflammation
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Local Production: LPS injection in rat air pouches elevates IL-1α (peaking at 1387 ± 598 pg/mL) alongside fever onset .
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Regulation: Delayed IL-1 receptor antagonist (IL-1ra) release modulates inflammatory responses .
Cellular Senescence
Product Specs
Interleukin-1 alpha, IL-1 alpha.
MGSSHHHHHH SSGLVPRGSH MGSSAPHSFQ NNLRYKLIRI VKQEFIMNDS LNQNIYVDMD
RIHLKAASLN DLQLEVKFDM YAYSSGGDDSKYPVTLKVSN TQLFVSAQGE DKPVLLKEIP
ETPKLITGSE TDLIFFWEKI NSKNYFTSAA FPELLIATKE QSQVHLARGL PSMIDFQIS.
Product Science Overview
The recombinant form of rat IL-1α, tagged with a His-tag, is produced in Escherichia coli (E. coli). This recombinant protein is a single, non-glycosylated polypeptide chain containing 179 amino acids, with a molecular mass of approximately 20.2 kDa . The His-tag, consisting of 23 amino acids, is fused to the N-terminus of the protein, facilitating its purification through affinity chromatography techniques .
IL-1α is a potent mediator of the inflammatory response. It acts as an endogenous pyrogen, meaning it can induce fever by affecting the hypothalamus. Additionally, IL-1α stimulates the release of prostaglandins and collagenase from synovial cells, contributing to the inflammatory process . The biological activity of recombinant rat IL-1α has been measured in cell proliferation assays using D10.G4.1 mouse helper T cells, with an effective dose (ED50) of less than or equal to 20 pg/ml .
Recombinant IL-1α is widely used in research to study its role in inflammation and immune responses. It is also utilized in various assays, including enzyme-linked immunosorbent assays (ELISA) and cell proliferation assays, to investigate its biological functions and interactions with other cytokines and cellular receptors .
For optimal stability, recombinant rat IL-1α should be stored at 4°C if it will be used within 2-4 weeks. For longer-term storage, it is recommended to store the protein at -20°C, with the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) to prevent degradation. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .
