IL1RAPL1 Antibody
Description
Definition and Target Profile
IL1RAPL1 (Interleukin-1 Receptor Accessory Protein-Like 1) is a transmembrane protein encoded by the IL1RAPL1 gene on the X chromosome. It regulates synapse formation, dendritic spine development, and AMPA receptor trafficking in neurons . Mutations in IL1RAPL1 are linked to X-linked intellectual disability (XLID) and autism .
IL1RAPL1 antibodies target specific epitopes of this protein, facilitating its detection in experimental models. Canonical IL1RAPL1 has a molecular weight of ~80 kDa, with isoforms detected in human, mouse, rat, and other species .
Research Applications
IL1RAPL1 antibodies are critical in studying:
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Synaptic Mechanisms: IL1RAPL1 interacts with Mcf2l and RhoA-ROCK pathways to regulate excitatory synapse formation in cortical neurons . Antibodies confirmed IL1RAPL1’s colocalization with synaptic markers like VGLUT1 .
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Disease Models: Mutations (e.g., Δex6, C31R) disrupt IL1RAPL1’s interaction with PTPδ, impairing synaptogenesis in intellectual disability models . Antibodies detected mislocalization and instability of mutant proteins .
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Signaling Pathways: IL1RAPL1 antibodies identified interactions with PKCε, Rasal1, and PLCβ1, highlighting its role in JNK pathway activation .
Research Findings
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Synaptogenesis: Overexpression of IL1RAPL1 in cortical neurons increased VGLUT1-positive excitatory synapses, an effect blocked by RhoA-ROCK inhibitors .
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Pathogenic Mutations: Δex6 and C31R mutants failed to induce synaptic differentiation due to reduced PTPδ binding, confirmed via coimmunoprecipitation .
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Subcellular Localization: IL1RAPL1 antibodies verified membrane and cytoplasmic localization, with postsynaptic enrichment in hippocampal neurons .
Challenges and Considerations
Product Specs
Target Background
- Our study revealed that the expression of IL-1R8 significantly increased on in vitro-activated CD4+ T cells and was markedly higher on CD4+ T cells from allergic rhinitis patients than on cells from healthy controls. PMID: 29730558
- IL-1R8 acts as a checkpoint for NK cell maturation and effector function; its genetic blockade unleashes NK-cell-mediated resistance to hepatic carcinogenesis, hematogenous liver and lung metastasis, and cytomegalovirus infection in mice. PMID: 29072292
- Altered DNA methylation in IL1RAPL1 is implicated in the etiology of Bipolar disorder and Major Depressive disorder. PMID: 27440233
- The rs12007907 variant in the IL1RAPL gene was negatively associated with asthma and IL-13 production in Latin American children. PMID: 28120837
- Our study expands the molecular repertoire of IL1RAPL1 mutations in intellectual disability and highlights the need for more precise clinical descriptions to better define the related phenotype. PMID: 27470653
- Evidence suggests that a defect in IL1RAPL1, which controls excitatory synapse formation, results in an imbalance of excitation and inhibition, affecting various cerebral functions. PMID: 25864829
- Novel IL1RAPL1 mutations associated with intellectual disability impair synaptogenesis. PMID: 25305082
- The interaction of the IL1RAPL1 family of proteins with PTPdelta and RhoGAP2 reveals a pathophysiological mechanism of cognitive impairment associated with a novel type of trans-synaptic signaling. PMID: 21926414
- The IL1RAPL1 gene is a candidate gene for autism spectrum disorder, as mutations or deletions in the gene have been previously reported in individuals from families with ASD. PMID: 21491612
- Intragenic deletions in IL1RAPL1 are relevant to the pathogenesis of X-linked mental retardation. PMID: 21271657
- Crystal structure at 2.3-A resolution of the TIR domain of IL-1RAPL. PMID: 15123616
- Nearly all patients with deletions involving DAX1, but not DMD, had mental retardation if IL1RAPL1 was deleted. If ILIRAPLI & DMD were intact, patients with DAX1 deletions only rarely had normal development. PMID: 15300857
- This report confirms the role of the IL1RAPL1 gene in causing nonspecific mental retardation in males. PMID: 16470793
- The DMD gene and its immediately distal neighbor, the 1.8 Mb IL1RAPL1 gene, are abundantly expressed in normal brain but were dramatically underexpressed in every brain tumor cell line and xenograft. PMID: 18253029
- Combined data suggest that IL1RAPL1 affects human cognitive ability to some extent, particularly memory and concentration capabilities. PMID: 18467032
- The function of the truncated IL1RAPL1 protein in an autistic female with Asperger syndrome is severely altered in hippocampal neurons, as demonstrated by its effect on neurite outgrowth activity. PMID: 18801879
- IL1RAPL1 plays a significant role in the etiology of X-linked mental retardation. PMID: 19012350
